Results of immunostaining of thrombospondin-1 (TSP-1) in different matrix locations
| Normal cartilage (n=7) | Mild OA (n=7) | Moderate OA (n=7) | Severe OA (n=9) | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SZ | MZ | DZ | CC | SZ | MZ | DZ | CC | MZ | DZ | CC | MZ | DZ | CC | ||||
| TSP-1 protein interterritorial | −/+ | +/++ | + | − | + | + | + | − | + | + | − | + | − | − | |||
| TSP-1 protein pericellular | − | ++ | − | − | − | ++ | − | − | +++ | + | − | + | + | − | |||
| TSP-1 RNA expressing chondrocytes (%) | 5.6 (2.6) | 13.1 (4.7) | 4.9 (3.0) | 0.0 | 4.8 (1.1) | 14.8 (8.2) | 4.0 (2.8) | 0.0 | 28.5 (8.8)1-150 | 5.7 (2.7) | 1.3 (1.6) | 10.5 (10.3) | 5.0 (1.7) | 0.0 | |||
| CD36 positive chondrocytes (%) | 38.0 (23.4) | 20.2 (13.2) | 6.4 (3.5) | 0.86 (1.6) | 54.0 (26.4) | 26.5 (24.5) | 5.0 (5.45) | 1.75 (1.6) | 50.9 (14.8)1-150 | 36.3 (26.4)1-150 | 2.8 (1.8) | 80.0 (17.5)1-150 | 61.4 (35.5)1-150 | 4.6 (2.8)1-150 | |||
SZ = superficial zone; MZ = middle zone; DZ = deep zone; CC = calcified cartilage. Intensity of immunostaining is represented by − (absent) to ++++ (very strong). Percentages of TSP-1 RNA expressing chondrocytes and CD36 immunostained chondrocytes in different cartilage zones are given as means (SEM).
↵1-150 Indicates a significant difference in Student'st test compared with normal cartilage (p<0.05).