Reaction (net A450) with histone of serum samples containing true antihistone IgG antibody (HS-1, HS-2) of heat aggregated normal serum (heated NS) and of serum samples from patients with RA3-150 (R11– R27)
| Specimens | Enzyme conjugate specificity | ||||||
|---|---|---|---|---|---|---|---|
| Anti-Fcγ non-digested | Anti-F(ab')2(detects IgG and IgM) | Anti-IgA α chain | |||||
| Non-digested | Pepsin digested | Non-digested3-151 | Pepsin digested3-151 | ||||
| HS-1 | 1.25 | 1.50 | 1.43 | 0.07 | 0.00 | ||
| HS-2 | 1.00 | 1.74 | 1.68 | NA | 0.00 | ||
| Heated NS | 1.85 | 1.47 | 0.03 | 0.69 | 0.00 | ||
| R11 | NA | 1.24 | 0.03 | NA | 0.05 | ||
| R12 | 1.20 | 1.16 | 0.06 | 0.69 | 0.00 | ||
| R13 | NA | 0.85 | 0.00 | NA | 0.00 | ||
| R14 | 0.91 | 1.36 | 0.01 | NA | 0.00 | ||
| R15 | NA | 1.17 | 0.10 | NA | 0.00 | ||
| R16 | 0.47 | 1.56 | 0.08 | 0.55 | 0.00 | ||
| R17 | 0.45 | 0.69 | 0.09 | 0.35 | 0.00 | ||
| R18 | 0.39 | 0.64 | 0.05 | NA | 0.00 | ||
| R19 | 0.35 | 1.25 | 0.09 | NA | 0.00 | ||
| R20 | 0.88 | 1.23 | 0.00 | 0.40 | 0.02 | ||
| R21 | 0.45 | 0.70 | 0.07 | NA | 0.05 | ||
| R22 | NA | 0.93 | 0.08 | 0.39 | 0.02 | ||
| R23 | 0.44 | 0.95 | 0.09 | 0.41 | 0.00 | ||
| R24 | 0.58 | 0.77 | 0.05 | 0.34 | 0.00 | ||
| R25 | NA | 1.08 | 0.05 | 0.67 | 0.03 | ||
| R26 | 0.46 | 1.55 | 0.02 | 0.43 | 0.01 | ||
| R27 | 0.83 | 0.95 | 0.11 | NA | 0.01 | ||
↵3-150 Specimens were selected if the A450 with anti-Fcγ was >0.35 or if the A450 with anti-IgM or anti-IgA was above the upper limit of normal.
↵3-151 Under the same conditions, using rabbit IgG as an antigen, the reduction due to pepsin digestion was less than 15%—that is, the IgA was pepsin resistant.
NA = not applicable (when A450 was within normal range).