Metabolism of [3H]-25-(OH)D3 by synovial fibroblasts and macrophages incubated separately and in co-culture
| Cell type | Metabolite synthesis (pmol/incubation/24 h) | |||
|---|---|---|---|---|
| 24,25-(OH)2D3 | 1,25-(OH)2D3 | 1,24,25-(OH)3D3 | Aqueous phase | |
| Macrophages (untreated) | nd | 12.1 (4.6) | nd | 1.5 (0.1) |
| IFNγ pre-treated macrophages | nd | 34.6 (3.0) | 1.3 (0.5) | 1.3 (0.3) |
| F733 (untreated) | 10.9 (0.8) | nd | nd | 0.7 (0.1) |
| Co-culture | 7.1 (0.2) | 12.4 (0.3) | 6.7 (0.0) | 5.4 (0.2) |
Results are expressed as mean (SEM), n=3; nd=none detected. The co-culture incubation consisted of F733 (untreated) and IFNγ pre-treated macrophages.