Conditions | ICAM-1 | |||
---|---|---|---|---|
4 hours | 8 hours | 24 hours | 48 hours | |
Mouse IgG1 PE control | 23 (6) | 16 (9) | 25 (7) | 20 (8) |
HUVEC+ media | 262 (12) | 656 (44) | 52 (38) | 555 (41) |
HUVEC+IL1 | 1033 (176) | 2922 (176) | 871 (99) | 1213 (109) |
HUVEC+IL1+TWH 50 ng/ml | 266 (38)*** | 474 (27)*** | 206 (25)** | 553 (39)* |
HUVEC+IL1+TWH 5 ng/ml | 841 (166) | 2048 (162) | 794 (87) | 876 (107) |
HUVEC+IL1+TWH 0.5 ng/ml | 778 (181) | 2104 (197) | 556 (124) | 1218 (98) |
HUVEC+IL1+TTD 10 μg/ml | 660 (115)* | 1695 (133)* | 554 (62)* | 636 (58)** |
HUVEC+IL1+TTD 1 μg/ml | 854 (149) | 1973 (194) | 588 (182) | 1348 (97) |
HUVEC+IL1+TTD 0.1 μg/ml | 1046 (98) | 1936 (181) | 787 (108) | 1209 (104) |
Effect on expression of ICAM-1 by HFB. Cells (5×106) were incubated with IL1α (10 ng/ml) in the presence or absence of herbal medicines for 4–24 hours. Data (fluorescence intensity) were presented as mean (SD) of duplicate assay in triplicate experiments. *p<0.05, **p<0.01, ***p<0.005 compared with HFB+IL1 by Mann-Whitney U test. Abbreviations as in table 1.