Table 2

Sequences of PCR primers, length of PCR product, optimal annealing temperature, sequence accession number from the NCBI Entrez search system and source of positive control

		Primers (5′-3′)		Template bp		Annealing °C	Sequence AC number	Positive control
		sense	antisense	Template bp		Annealing °C	Sequence AC number	Positive control
MMP-1	CTGAAGGTGATGAAGCAGCC	AGTCCAAGAGAATGGCCGAG	428	62	X05231	fibroblast
MMP-2	GCGACAAGAAGTATGGCTTC	TGCCAAGGTCAATGTCAGGA	390	62	J03210	fibroblast
MMP-3	CTCACAGACCTGACTCGGTT	CACGCCTGAAGGAAGAGATG	294	58	J03209	fibroblast
MMP-7	GTGGTCACCTACAGGATCGT	ACCATCCGTCCAGCGTTCAT	282	62	Z11887	breast cancer
MMP-8	ATGGACCAACACCTCCGCAA	GTCAATTGCTTGGACGCTGC	532	64	J05556	bone marrow
MMP-9	CGCAGACATCGTCATCCAGT	GGATTGGCCTTGGAAGATGA	406	62	J05070	keratinocyte
MMP-10	GTCCTTCGATGCCATCAGCA	CTTGCTCCATGGACTGGCTA	380	62	X07820	fibroblast
MMP-11	CAGGTGGCAGCCCATGAATT	GTACTGAGCACCTTGGAAGA	456	58	X57766	breast cancer
MMP-12	CCACTGCTTCTGGAGCTCTT	GCGTAGTCAACATCCTCACG	367	62	L23808	breast cancer
MMP-13	CTATGGTCCAGGAGATGAAG	AGAGTCTTGCCTGTATCCTC	390	62	X75308	breast cancer
MMP-14	CAACACTGCCTACGAGAGGA	GTTCTACCTTCAGCTTCTGG	380	60	D26512	breast cancer
MMP-15	GCATCCAGAACTACACGGAG	TACCGTAGAGCTGCTGGATG	474	62	Z48482	breast cancer
MMP-16	TGTACCTGACCAGACAAGAG	AGTGTCCATGGCTCATCTGA	384	58	D50477	breast cancer
MMP-17	GACCTGTTTGCAGTGGCTGT	ACGATCTTGTGGTCGCTGGT	473	64	X89576	breast cancer
MMP-19	CAGGCTCTCTATGGCAAGAA	GAGCTGCATCCAGGTTAGGT	397	62	X92521	breast cancer
MMP-20	GACCAGACCACAATGAACGT	GTCCACTTCTCAGGATTGTC	374	62	Y12779	odontoblast
β-actin	CACCTTCTACAATGAGCTGC	AGGCAGCTCGTAGCTCTTCT	466	62	X00351	breast cancer