Patient | DRB1 genotype | Expansion | Subset | Number sequenced | Number clonal | CDR3 | BJ segment |
---|---|---|---|---|---|---|---|
R1 | *13/*16 | Vβ1 | CD4+ | 13 | 7 | CASTPGVKMNTEAF FGQG | 1S1 |
R4 | *0401/*0404 | Vβ8 | CD4+ | 21 | 10 | CASGMTGQGTDTQY FGPG | 2S3 |
R2 | *0408/*0101 | Vβ11 | CD8+ | 10 | 6 | CASVWTTHEF FGPG | 2S1 |
R3 | *0401/*0401 | Vβ5.1 | CD8+ | 9 | 8 | CASSWDKAYEQY FGPG | 2S7 |
R3 | *0401/*0401 | Vβ5.2 | CD8+ | 16 | 2 | ||
R4 | *0401/*0404 | Vβ5.2 | CD8+ | 12 | 6 | CASSLIDSILTDTQY FGPG | 2S3 |
R4 | *0401/*0404 | Vβ18 | CD8+ | 20 | 9 | CASSPSRQISGANVLT FGAG | 2S6 |
R5 | *0401/*11 | Vβ11 | CD8+ | 12 | 4 | CASSFSRSTDTQY FGPG | 2S3 |
R5 | *0401/*11 | Vβ13.1 | CD8+ | 17 | 9 | CASSQGRTSVTEQY FGPG | 2S7 |
R6 | *0401/*11 | Vβ6 | CD8+ | 14 | 12 | CASSLTGTEHNEQF FGPG | 2S1 |
Ten Vβ-Cβ amplification products displaying a dominant peak in the spectratype were subcloned and sequenced. The number of subclones sequenced from each sample is indicated. A minimum of three identical sequences was required to demonstrate the unequivocal presence of a clonal expansion. This criterion was satisfied in nine of 10 samples. The inferred amino acid sequence of the CDR3 loop is displayed in bold single letter amino acid code.