Monoclonal antibodies and secondary reagents used
| Antibody | Isotype | Source |
|---|---|---|
| CD3-BIO | IgG1 | Dako (High Wymcombe, Buckinghamshire, UK) |
| CD4-BIO | IgG1 | Dako |
| CD4-FITC | IgG1 | Dako |
| CD4-PE | IgG1 | Dako |
| CD8-PE | IgG1 | Dako |
| CD25-FITC | IgG1 | Dako |
| CD69-FITC | IgG1 | Dako |
| HLA-DR-FITC | IgG1 | Serotec (Kiddlington, Oxfordshire, UK) |
| TCRαβ-FITC | IgG1 | Becton Dickinson (Cowley, Oxfordshire, UK) |
| TCRγδ-FITC | IgG1 | Becton Dickinson |
| CD45RA-FITC | IgG1 | Serotec |
| CD45RO-FITC | IgG2a | Serotec |
| Secondary reagents | ||
| Streptavidin-RED670 | — | Life Technologies (Paisley, Scotland) |
| Negative controls | ||
| IgG1-FITC | — | Dako |
| IgG1-PE | — | Dako |
| IgG1-BIO | — | Dako |
| IgG2a-FITC | — | Dako |