A Histopathological-histochemical scoring parameters: | Score (12) | |
---|---|---|
1 | Cellularity | |
Normal cellularity | 0 | |
<10 % of acellular cartilage | 1 | |
10–50% of acellular cartilage | 2 | |
>50% of acellular cartilage | 3 | |
2 | Loss of matrix (erosion of the articular cartilage) | |
No loss | 0 | |
<10% of eroded regions | 1 | |
10–25% of eroded regions | 2 | |
>25% of eroded regions | 3 | |
3 | Clustering of chondrocytes (propagating cells in groups) | |
No clustering | 0 | |
<10% of chondrocytes in clusters | 1 | |
10–25% of chondrocytes in clusters | 2 | |
>25% of chondrocytes in clusters | 3 | |
4 | Adhesions (pannus) | |
No adhesions | 0 | |
Covering only the margins of cartilage | 1 | |
Covering<50% of articular surface | 2 | |
Covering >50% of articular surface | 3 | |
51-150 | Orthochromasia with Alcian blue | |
Normal | 0 | |
Slight and patchy loss of staining (×1–2) | 1 | |
Moderate loss (×2–3) | 2 | |
Severe loss (×4–5) | 3 | |
6 | Significance of synovitis reflected by inflammation of synovial membrane | |
Normal synovia | 0 | |
Mild cellular infiltration of synovia | 1 | |
Moderate cellular infiltration of synovia | 2 | |
Severe cellular infiltration of synovia | 3 | |
B Additional histopathological cartilage scoring: | ||
1 | Vascular elements crossing of cartilage-subchondral bone tidemark | |
2 | Height of articular cartilage zone | |
3 | Enchondral bone marrow cavities |
↵1-150 Alcian blue was used for staining acid mucopolysaccharides instead of Safranin-O in Salter’s study.