Abnormal numbers of cytokine producing cells in patients with polymyositis and dermatomyositis

Clin Exp Rheumatol. 1996 Sep-Oct;14(5):485-91.

Abstract

Objective: To examine the constitutive pattern of cytokine production in patients with polymyositis (PM) and dermatomyositis (DM).

Methods: PBMC secreting the type 1 cytokines IL-2 and IFN gamma and the type 2 cytokines IL-6 and IL-10 were monitored by ELIspot assay.

Results: The frequency of IFN gamma-secreting PBMC was reduced 2-20 fold in patients with PM and DM when compared to normal controls (p < 0.01). This change correlated inversely with disease activity (p < 0.01). PM and DM were also characterized by increased numbers of cells secreting IL-10. Patients with DM had fewer IL-2 (p < 0.03) and more IL-6 (p < 0.006) secreting cells than normal controls or patients with other myopathies.

Conclusions: Abnormal patterns of cytokine production characterize patients with PM and DM.

MeSH terms

  • Adult
  • Cell Count
  • Cytokines / biosynthesis*
  • Dermatomyositis / diagnosis*
  • Humans
  • Leukocytes, Mononuclear / metabolism
  • Middle Aged
  • Polymyositis / diagnosis*

Substances

  • Cytokines