Abstract
The effects of lupus anticoagulant (LAC) on cultured human umbilical vein endothelial cells were studied. All five monoclonal antibodies from a patient with systemic lupus erythematosus (SLE), as well as plasma samples with LAC activity from six SLE patients, induced apoptosis. Anti-annexin V IgG also induced apoptosis. Since monoclonal antibodies and plasma from SLE patients had an affinity for annexin V, an endothelial apoptosis pathway mediated by annexin V was suggested as the molecular pathogenesis of the hemostatic derangement associated with LAC.
MeSH terms
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Animals
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Annexin A5 / analysis
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Annexin A5 / immunology
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Annexin A5 / physiology*
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Antibodies / pharmacology
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Antibodies, Monoclonal / isolation & purification
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Antibodies, Monoclonal / pharmacology*
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Apoptosis / drug effects*
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Autoantibodies / isolation & purification
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Autoantibodies / pharmacology*
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B-Lymphocytes / immunology
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Cells, Cultured
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Clone Cells
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DNA Damage
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / physiology*
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Endothelium, Vascular / ultrastructure
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Enzyme-Linked Immunosorbent Assay
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Humans
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Immunoglobulin G / isolation & purification
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Immunoglobulin G / pharmacology
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Immunoglobulin M / isolation & purification
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Immunoglobulin M / pharmacology
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Lupus Coagulation Inhibitor / pharmacology*
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Lupus Erythematosus, Systemic / immunology
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Microscopy, Electron
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Rabbits
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Umbilical Veins
Substances
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Annexin A5
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Antibodies
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Antibodies, Monoclonal
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Autoantibodies
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Immunoglobulin G
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Immunoglobulin M
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Lupus Coagulation Inhibitor