Enhancement of endothelial cell E-selectin expression by sera from patients with active Behçet's disease: moderate correlation with anti-endothelial cell antibodies and serum myeloperoxidase levels

Clin Immunol. 1999 Jun;91(3):330-7. doi: 10.1006/clim.1999.4687.

Abstract

We studied the in vitro E-selection expression of endothelial cells treated with sera from patients with Behçet's disease (BD) and factors (anti-endothelial cell antibodies, anti-neutrophil cytoplasmic antibodies, cytokines, and myeloperoxidase (MPO) that may contribute to adhesion molecule expression. A total of 21 patients with BD and 27 healthy controls were studied. In vitro E-selectin endothelial cell expression was investigated by ELISA after HUVEC incubation with sera or purified IgG from patients with BD and controls. Increased E-selectin expression was observed when endothelial cells were incubated with sera from patients with active disease or from patients with circulating anti-endothelial cell antibodies and high levels of plasma myeloperoxidase. Abnormalities of endothelial cell function have been suggested to play a role in the occurrence of vascular damage in BD. Our findings suggest that anti-endothelial cell antibodies and neutrophil hyperactivity, as inferred from the high plasma MPO levels in patients with active disease, may explain endothelial cell activation and neutrophil accumulation in vascular lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antibodies, Antineutrophil Cytoplasmic / blood
  • Autoantibodies / blood*
  • Behcet Syndrome / blood*
  • Behcet Syndrome / enzymology
  • Behcet Syndrome / immunology*
  • Cells, Cultured
  • Cytokines / blood
  • E-Selectin / blood
  • E-Selectin / metabolism*
  • Endothelium, Vascular / immunology
  • Female
  • Humans
  • Immunoglobulin G / blood
  • In Vitro Techniques
  • Male
  • Middle Aged
  • Neutrophils / enzymology
  • Peroxidase / blood*
  • Peroxidase / immunology

Substances

  • Antibodies, Antineutrophil Cytoplasmic
  • Autoantibodies
  • Cytokines
  • E-Selectin
  • Immunoglobulin G
  • anti-endothelial cell antibody
  • Peroxidase