Summary
The presence of proliferating cells has been sought in the synovium of rheumatoid arthritic (RA) and osteoarthritic (OA) joints using the monoclonal antibody Ki67, which marks a nuclear antigen present in all stages of the cell cycle apart from Go. Synovia were studied from 21 RA and 14 OA cases using the indirect immunoperoxidase technique. Double-staining was performed on 18 RA and 17 OA synovia with the simultaneous labelling of lysozyme (muramidase) by the immuno-alkaline phosphatase method and with Ki67 by the indirect immunoperoxidase method.
Most of the RA and OA synovia showed an absence of Ki67-positive cells in the intimal cell layer. Three RA and four OA synovia showed no more than ten proliferating cells in the whole of the intimal layer examined. Similar results were obtained when double-labelling was performed. Eight RA and six OA synovia showed the presence of occasional Ki67-positive cells in the intimal layer. The total number of intimal cells was measured for each histological section, and the proliferation index calculated as the percentage of total cells in the intimal layer showing Ki67-positive staining. This varied between 0.03% and 0.0033% (between 1 : 2800 and 1 : 30 000 cells). In contrast, there were plentiful Ki67-positive cells present in the lymphocytic infiltrate and around blood vessels in the RA synovia and in the synovial infiltrate, where present, in the OA cases. The results provide further support for the suggestion that the intimal cell layer of the synovium is predominantly composed of macrophages, with some fibroblasts, and that the macrophages have not arisen locally by cell division (hyperplasia) but have migrated from the underlying synovial blood vessels.
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References
Barland P, Novikoff AB, Hamerman D (1962) Electron microscopy of the human synovial membrane. J Cell Biol 14:207–220
Bierther MF, Wegner KW, Fackeldey HD (1972) The fine structure of normal synovium in the dog as compared with the synovium after transplantation of a total knee joint. Z Rheumaforsch 31:262–272
Ghadially FN (1983) The fine structure of synovial joints. Butterworths, London
Gardner DL (1972) The pathology of rheumatoid arthritis. Arnold, London
Fassbender HG (1975). Pathology of rheumatic diseases. Springer, Berlin Heidelberg New York
Coulton LA, Henderson B, Bitensky L, Chayen J (1980) DNA synthesis in the human rheumatoid and non-rheumatoid synovial lining. Ann Rheum Dis 39:241–247
Gerdes J, Schwab H, Lemke H, Stein H (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 31:13–20
Gerdes J, Lemke H, Baisch H, Wacker H-H, Schwab U, Stein H (1984) Cell cycle analysis of cell proliferation — associated human nuclear antigen defined by the monoclonal antibody Ki67. J Immunol 133:1710–1715
Palmer DG, Selvendran Y, Allen C, Revell PA, Hogg N (1985) Features of synovial membrane identified with monoclonal antibodies. Clin Exp Immunol 59:529–538
Hogg N, Palmer DG, Revell PA (1985) Mononuclear phagocytes of normal and rheumatoid synovial membrane identified by monoclonal antibodies. Immunology 56:673–681
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Mohr W, Beneke G, Mohing W (1975) Proliferation of synovial lining cells and fibroblasts. Ann Rheum Dis 34:219–224
Mapp PI, Revell PA (1987) The ultrastructural localisation of muramidase in the human synovial membrane. Ann Rheum Dis 46:30–37
Mapp PI (1987) Studies on the intimal cells of the synovial membrane. PhD thesis, University of London
Edwards JCW, Willoughby DA (1982) Demonstration of bone marrow derived cells in synovial lining by means of giant intracellular granules as genetic markers. Ann Rheum Dis 41:177–182
Dreher R (1982) The origin of synovial type A cells during inflammation: an experimental approach. Immunobiology 161:232–245
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Lalor, P.A., Mapp, P.I., Hall, P.A. et al. Proliferative activity of cells in the synovium as demonstrated by a monoclonal antibody, Ki67. Rheumatol Int 7, 183–186 (1987). https://doi.org/10.1007/BF00541375
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DOI: https://doi.org/10.1007/BF00541375