Regular Article
Pulsating Fluid Flow Increases Prostaglandin Production by Cultured Chicken Osteocytes—A Cytoskeleton-Dependent Process

https://doi.org/10.1006/bbrc.1996.1131Get rights and content

Abstract

It has been postulated that the transduction of mechanical stress signals to bone cells occurs via loading-dependent flow of interstitial fluid through the lacuno-canalicular network of bone. We have shown earlier that chicken osteocytes release enhanced amounts of prostaglandin E2after 1 h treatment with pulsating fluid flow (PFF, 0.5 ± 0.02 Pa, 5 Hz). Here we study the acute response to PFF on three cell populations derived from fetal chick calvariae, namely periosteal fibroblasts (PF), an osteoblast and osteocyte containing population (OBmix), and osteocytes (OCY), and the involvement of the actin-cytoskeleton in this process. All three cell populations rapidly (OCY: within 5 min, OBmix, PF: within 10 min) increased their release of prostaglandins E2and I2in response to PFF, but the response by OCY was 2–4 times higher than that by OBmix or PF. Disruption of the actin-cytoskeleton by cytochalasin B completely abolished the response. We conclude that osteocytes are more sensitive to fluid shear stress than immature bone cells, and that the actin-cytoskeleton is involved in the response to fluid flow.

References (0)

Cited by (0)

Abbreviations used: AA, arachidonic acid; PFF, pulsating fluid flow; PGE2, prostaglandin E2; PGI2, prostaglandin I2; OCY, osteocytes; PF, periosteal fibroblasts; OBmix, osteoblast- and osteocyte-containing population.

View full text