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Basic and translational research
Concise report
Ankylosing spondylitis is associated with the anthrax toxin receptor 2 gene (ANTXR2)
  1. T Karaderi1,
  2. S M Keidel1,
  3. J J Pointon1,
  4. L H Appleton2,
  5. M A Brown3,
  6. D M Evans3,4,
  7. B P Wordsworth1,2
  1. 1National Institute for Health Research Oxford Comprehensive Biomedical Research Centre, University of Oxford, Oxford, UK
  2. 2National Institute for Health Research Oxford Musculoskeletal Biomedical Research Unit, University of Oxford, Oxford, UK
  3. 3University of Queensland Diamantina Institute, Translational Research Institute, Brisbane, Queensland, Australia
  4. 4MRC Integrative Epidemiology Unit, University of Bristol, Bristol, UK
  1. Correspondence to Professor B P Wordsworth, National Institute for Health Research Musculoskeletal Biomedical Research Unit, Nuffield Orthopaedic Centre, Windmill Rd, Headington, Oxford OX3 7LD, UK; paul.wordsworth{at}ndorms.ox.ac.uk

Abstract

Objectives ANTXR2 variants have been associated with ankylosing spondylitis (AS) in two previous genome-wide association studies (GWAS) (p∼9×10−8). However, a genome-wide significant association (p<5×10−8) was not observed. We conducted a more comprehensive analysis of ANTXR2 in an independent UK sample to confirm and refine this association.

Methods A replication study was carried out with 2978 cases and 8365 controls. Then, these were combined with non-overlapping samples from the two previous GWAS in a meta-analysis. Human leukocyte antigen (HLA)-B27 stratification was also performed to test for ANTXR2-HLA-B27 interaction.

Results Out of nine single nucleotide polymorphisms (SNP) in the study, five SNPs were nominally associated (p<0.05) with AS in the replication dataset. In the meta-analysis, eight SNPs showed evidence of association, the strongest being with rs12504282 (OR=0.88, p=6.7×10−9). Seven of these SNPs showed evidence for association in the HLA-B27-positive subgroup, but none was associated with HLA-B27-negative AS. However, no statistically significant interaction was detected between HLA-B27 and ANTXR2 variants.

Conclusions ANTXR2 variants are clearly associated with AS. The top SNPs from two previous GWAS (rs4333130 and rs4389526) and this study (rs12504282) are in strong linkage disequilibrium (r2≥0.76). All are located near a putative regulatory region. Further studies are required to clarify the role played by these ANTXR2 variants in AS.

  • Ankylosing Spondylitis
  • Inflammation
  • Gene Polymorphism
  • Epidemiology
  • Spondyloarthritis

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

https://doi.org/10.1136/annrheumdis-2014-205643

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