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Targeting Th2-typified immune responses to prevent immunopathology in rheumatic diseases: belittled therapeutic strategies?
  1. Frederique M Moret1,
  2. Timothy R D J Radstake1,2,
  3. Joel A G van Roon1,2
  1. 1Department of Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
  2. 2Center for Molecular and Clinical Immunology, Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
  1. Correspondence to Dr Joel A G van Roon, Department of Rheumatology and Clinical Immunology, University Medical Center Utrecht, PO Box 85500, Utrecht 3508 GA, The Netherlands; j.vanroon{at}umcutrecht.nl

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Although Th2-associated immune responses have been well defined in atopic diseases and parasitic infections, their potential contribution to immunopathology in rheumatic diseases has scarcely been recognised. This is probably related to the clear predominance of Th1/Th17 over Th2 cells in a number of rheumatic diseases, such as rheumatoid arthritis (RA), psoriatic arthritis and primary Sjögren's syndrome (pSS). Also, whereas a clear role for Th1/Th17 activity in immunopathology has been clearly demonstrated in these diseases, Th2-related phenomena such as IgE production are hardly detected. Indeed, Th2-associated atopic conditions and Th2 cytokines like interleukin (IL)-4 and IL-10 were shown to inhibit Th1-induced inflammatory responses in these diseases.1 ,2 As a consequence the understanding of Th2-associated mediators, other than IL-4 and IgE, in these and other rheumatic conditions and the potential to target these have been underevaluated. However, recent data show that immune activation by typical Th2-associated pathways, such as mast cell activation and histamine-induced responses, or Th2-typified key regulatory molecules, such as thymic stromal lymphopoietin (TSLP), IL-33 and IL-13, contribute to inflammation and immunopathology in several rheumatic diseases.

Mast cells and pathways driving histamine release: targets in rheumatic diseases

Mast cells have been shown to have a detrimental role in allergic reactions and parasitic infections. In addition, mast cells have been shown to be increased at inflammatory sites in a plethora of rheumatic conditions, including RA, pSS, systemic sclerosis (SSc), spondyloarthropathies (SpA), dermatomyositis and eosinophilia-myalgia syndromes.3–8 Mast cells have also been shown to be important mediators in different experimental inflammatory (arthritis) models by secretion of proinflammatory mediators such as tumour necrosis factor α (TNFα), IL-6, IL-8 and IL-17. In addition, inhibitors of histamine release such as salbutamol and cromolyn were shown to prevent joint destruction in immune complex-mediated arthritis.9 ,10 Recent data suggest that targeting of mast cell-associated pathways prevents inflammatory responses driven by the innate and acquired immune system. One such pathway may be prevention of immune activation through blockade of the histamine H4 receptor (H4R). The human H4R expression is largely restricted to cells of the human immune system (eg, mast cells, eosinophils, monocytes, dendritic cells, T cells) and mediates several proinflammatory effects on these cell types, including cytokine secretion and chemotaxis.11 Cowden et al12 add new insights into the mechanism of this receptor, demonstrating that specific targeting of the H4R by an orally administered receptor antagonist prevents joint inflammation and inflammation-induced destruction of cartilage and bone. Using different experimental arthritis models, they demonstrate that innate effector cells, such as neutrophils and monocytes (collagen antibody-induced arthritis model), and acquired effector cells, like Th17 cells (collagen-induced arthritis model), play critical roles. Previous studies using antagonists of the H1 and H2 receptors have not been successful in treating autoimmune diseases; however, these antagonists do not affect the H4R.13 These findings indicate new therapeutic opportunities in RA, but also many other rheumatic diseases associated with increases in histamine and (upregulated) expression of H4R, in particular given its restricted expression to immune cells.13

Histamine levels have been shown to be enhanced at inflammatory sites in several (rheumatic) autoimmune diseases, including multiple sclerosis, inflammatory bowel disease, RA, ankylosing spondylitis, psoriatic arthritis and SSc. Triggers of histamine release by mast cells include IgE-dependent pathways but also many IgE-independent pathways. Increased IgE complexes and total serum IgE levels in various autoimmune diseases such as RA, pSS and systemic lupus erythematosus (SLE) are present in only a minority of patients (∼10–30% of the patients). To what extent antigen-specific IgE antibodies trigger mast cell activation and histamine release in rheumatic diseases remains to be demonstrated but this might play a role in at least a subgroup of patients. Activation of mast cells by cross-linking of the high-affinity IgE receptor results in high TSLP and IL-33 secretion by these cells.14 ,15 Both cytokines are upregulated in several rheumatic diseases and induce activation of mast cells,3 ,16 potentially contributing to a positive feedback loop in chronic inflammation by enhancing release of histamine and other proinflammatory mediators, like TNFα and IL-17.

IgE-independent mechanisms that have been described to trigger degranulation and histamine release include immunoglobulin complexes, complement and free light chains.10 ,17 Toll-like receptor (TLR)-mediated histamine release has also been suggested, although the data are not consistent,18 which is in contrast to data on numerous inflammatory mediators that are released upon TLR-induced mast cell activation (for instance, interferons and TNFα).19 Despite the inconsistency of results for direct histamine release by TLR ligation, TLR-induced activation of fibroblasts, either directly or indirectly through release of proinflammatory cytokines (including IL-1β and TNFα), induces release of cytokines, which like IL-33 and TSLP enhance histamine release.20–22 In addition, recently it was demonstrated that TLR-induced activation of neutrophils promotes histamine production via a PI3 kinase-dependent mechanism.23 Likewise, it has been shown that cell types such as dendritic cells contribute to increased histamine levels during inflammation, although these cells may induce lower levels.13 It is interesting that activation of cells through triggering of the H4R requires much lower levels of histamine than with the H1–3 receptors (∼100–1000-fold).13 Collectively, the increased numbers of mast cells, enhanced levels of histamine and numerous triggers to activate these and other cells to release histamine suggest that new approaches targeting histamine-associated pathways, as indicated by Cowden et al,12 and including inhibition of mast cell activation, hold promise in various rheumatic diseases.

Histamine H4 receptor: an example of targetable th2 typified amplifiers in immunopathology of rheumatic diseases

Recently, in (models for) RA, SpA, SSc and SLE, other pathways that have been typified as atopy/Th2-associated immune responses, including those driven by TSLP, IL-33 and IL-13, have been identified as targets for treatment in rheumatic diseases, extending the data of Cowden et al.12 Strongly enhanced levels of these cytokines facilitating proinflammatory responses are present in a much higher percentage of patients than is the case, for example, for IgE.

Recently, increased TSLP levels were reported in the synovial fluid of patients with RA in comparison with patients with osteoarthritis.21 ,24 TSLP was shown to activate enhanced numbers of CD1c-expressing myeloid dendritic cells to robustly induce Th1 and Th17 activity in addition to Th2 activity.24 ,25 In support of a role in RA, TSLP in mice enhanced the severity of collagen-induced arthritis, associated with enhanced joint inflammation and destruction of cartilage and bone. Conversely, mice deficient in TSLP receptor displayed strongly reduced arthritis severity and immunopathology, associated with strong inhibition of evident Th17 activity and modest Th2 activity.26 In analogy to histamine, this illustrates that TSLP may be a target in atopic diseases, and also in autoimmune disease like RA, predominantly regulating Th17 activity.

Mediators like TSLP were also shown to play a role in rheumatic diseases apart from RA in which Th2/Th17 responses seem to be more prominent. Recently, it was demonstrated that in addition to epithelial cells and fibroblasts, mast cells in human SSc skin and lung fibrosis and in the bleomycin model of scleroderma overexpressed TSLP,22 ,27 which in its turn can activate mast cells.16 In cultured skin fibroblasts, TSLP expression was induced upon TLR triggering. TSLP in the skin of patients with SSc induced profibrotic genes and intracellular signalling that overlap with those induced by IL-13 and transforming growth factor (TGF) β, both pivotal mediators in SSc.27 In addition, TSLP was demonstrated to induce TGFβ-dependent fibrosis induction. Furthermore, in TSLP receptor-deficient mice, bleomycin-induced fibrosis was significantly reduced in parallel with significantly reduced local expression of IL-13.22 Finally, TSLP might also be a link to IL-33 expression, which induces IL-13-dependent cutaneous fibrosis,28 as levels of IL-33 are raised in patients with SSc correlating with the extent of skin sclerosis and severity of pulmonary fibrosis.29 Since TSLP has the capacity to enhance Th2 activity associated with IL-33 and IL-13 production in protease-allergen-induced airways inflammation,30 this indicates a potential TSLP-induced axis in SSc, contributing to fibrosis.

Other rheumatic diseases in which enhanced expression of IL-33 and IL-13 has been demonstrated include pSS, SpA and RA.20 ,31 ,32 Serum levels of IL-33 were significantly raised in patients with pSS, especially in patients with interstitial lung disease. IL-33 significantly correlated with rheumatoid factor and anti-SSB autoantibody levels in pSS.31 Increased levels of IL-33 and IL-13 were also detected in the joints of patients with RA and have been shown to contribute to synovial fibroblast hyperplasia.20

The study by Cowden et al12 (this issue of ARD) demonstrates the beneficial effects of blockade of the H4R to prevent innate and acquired immune responses and thereby immunopathology in experimental arthritis. Recent data demonstrate that targeting of other Th2-associated mediators, like TSLP, IL-33 and IL-13, prevents inflammation and immunopathological processes such as fibrosis. Collectively, these data emphasise that targets previously considered relevant in Th2-associated diseases, should be evaluated to prevent specific immunopathological processes in rheumatic diseases such as SSc, RA, pSS, SpA and SLE. Clinical trials will be required to document how such strategies might fit into the current armamentarium to treat these diseases.

References

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Footnotes

  • Contributors All authors contributed to the manuscript and approved the final manuscript.

  • Competing interests None.

  • Provenance and peer review Commissioned; externally peer reviewed.

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