Objectives: Both antibody and cell-mediated immune responses are involved in the defence against influenza. Although antibody responses are unaltered in Wegener’s granulomatosis (WG), the level of cell-mediated responses to vaccination could be deficient.
Methods: Twenty-five WG patients and healthy controls received subunit influenza vaccine. PBMCs were obtained before and one month after vaccination. Cell-mediated responses to A/H1N1 and A/H3N2 were assessed using interferon-γ (IFN-γ) ELISpot and intracellular cytokine staining for IFN-γ, tumour necrosis factor (TNF) and interleukin-2 (IL-2).
Results: Prior to vaccination, patients and controls showed similar recall responses to A/H1N1 and A/H3N2. Following vaccination, patients and controls showed similar levels of increase in spot-forming cells against A/H1N1 and A/H3N2. By flow cytometry, upon vaccination, proportions of cytokine-producing CD4+ T-cells increased in patients and controls for A/H1N1 and A/H3N2.
Conclusions: Cell-mediated responses to influenza vaccination in WG patients are comparable to those in healthy controls. Dutch Trials Register, NTR1130.