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Cell-mediated immune responses to influenza vaccination in Wegener's granulomatosis
  1. Albert Holvast (b.holvast{at}int.umcg.nl)
  1. University Medical Center Groningen, Netherlands
    1. Aalzen De Haan
    1. University Medical Center Groningen, Netherlands
      1. Sander Van Assen
      1. University Medical Center Groningen, Netherlands
        1. Coen A Stegeman
        1. University Medical Center Groningen, Netherlands
          1. Minke G Huitema
          1. University Medical Center Groningen, Netherlands
            1. Anke Huckriede
            1. University Medical Center Groningen, Netherlands
              1. Cornelis A Benne
              1. Laboratory for Infectious Diseases, Netherlands
                1. Johanna Westra
                1. University Medical Center Groningen, Netherlands
                  1. Abraham Palache
                  1. Solvay Pharmaceuticals, Netherlands
                    1. Jan Wilschut
                    1. University Medical Center Groningen, Netherlands
                      1. Cees G M Kallenberg
                      1. University Medical Center Groningen, Netherlands
                        1. Marc Bijl
                        1. University Medical Center Groningen, Netherlands

                          Abstract

                          Objectives: Both antibody and cell-mediated immune responses are involved in the defence against influenza. Although antibody responses are unaltered in Wegener’s granulomatosis (WG), the level of cell-mediated responses to vaccination could be deficient.

                          Methods: Twenty-five WG patients and healthy controls received subunit influenza vaccine. PBMCs were obtained before and one month after vaccination. Cell-mediated responses to A/H1N1 and A/H3N2 were assessed using interferon-γ (IFN-γ) ELISpot and intracellular cytokine staining for IFN-γ, tumour necrosis factor (TNF) and interleukin-2 (IL-2).

                          Results: Prior to vaccination, patients and controls showed similar recall responses to A/H1N1 and A/H3N2. Following vaccination, patients and controls showed similar levels of increase in spot-forming cells against A/H1N1 and A/H3N2. By flow cytometry, upon vaccination, proportions of cytokine-producing CD4+ T-cells increased in patients and controls for A/H1N1 and A/H3N2.

                          Conclusions: Cell-mediated responses to influenza vaccination in WG patients are comparable to those in healthy controls. Dutch Trials Register, NTR1130.

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