Objectives: To assess the possible association of KIR genes, specifically KIR3DL1, KIR3DS1 and KIR3DL2, with ankylosing spondylitis (AS).
Methods: 14 KIR genes were genotyped in 200 UK patients with AS and 405 healthy controls using multiplex PCR. Sequence-specific oligonucleotide probes were used to subtype 368 AS cases and 366 controls for 12 KIR3DL2 alleles. Differences in KIR genotypes and KIR3DL2 allele frequencies were assessed using the χ2 test.
Results: KIR3DL1 and KIR3DS1 gene frequencies were very similar in AS cases and controls (OR = 1.5, 95% confidence interval (CI) 0.8-3.0, and OR = 1.02, 95% CI 0.2-5.3, respectively). KIR3DL2 allele frequencies were not significantly different between AS cases and controls.
Conclusion: Neither the KIR gene content of particular KIR haplotypes nor KIR3DL2 polymorphisms contribute to AS.