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New autoantigens in rheumatoid arthritis: screening 8268 protein arrays with RA patients’ sera.
  1. Isabelle Auger (isabelle.auger{at}medecine.univ-mrs.fr)
  1. INSERM UMR 639, France
    1. Nathalie Balandraud (npbalandraud{at}yahoo.fr)
    1. INSERM UMR 639 and APHM, France
      1. Justyna Rak (justyna.rak{at}gmail.com)
      1. INSERM UMR 639, France
        1. Nathalie Lambert (nathalie.lambert{at}medecine.univ-mrs.fr)
        1. INSERM UMR 639, France
          1. Marielle Martin (marielle.martin{at}medecine.univ-mrs.fr)
          1. INSERM UMR 639, France
            1. Jean Roudier (jean.roudier{at}medecine.univ-mrs.fr)
            1. INSERM UMR 639 and APHM, France

              Abstract

              Objective: To identify new IgG autoantibodies in rheumatoid arthritis patients’ sera.

              Methods: We tested serum samples from 19 rheumatoid arthritis patients with given HLA-DR genotypes, from 7 spondylarthropathy patients, 2 lupus patients, 4 systemic sclerosis patients and 10 healthy individuals on 8268 human protein arrays.

              Results: We identified four antigens (PAD4, PKC beta 1, PIP4K2C and BRAF) that were recognized almost uniquely by sera from patients with RA on protein arrays. Using purified proteins, we confirmed that PAD4 and BRAF are recognized almost uniquely by RA patients.

              Conclusion: We identified PAD4 and BRAF as RA specific autoantigens.

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