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Detection, identification and in vivo treatment responsiveness of BMP activated cell populations in the synovium of rheumatoid arthritis patients
  1. Patrick CPM Verschueren (patrick.verschueren{at}uz.kuleuven.ac.be)
  1. University Hospitals Leuven, Belgium
    1. Rik J.U. Lories (rik.lories{at}uz.kuleuven.ac.be)
    1. Universitaire Ziekenhuizen Leuven, Belgium
      1. Melina Daans (melina.daans{at}med.kuleuven.be)
      1. Division of Rheumatology KU Leuven, Belgium
        1. Ivan Théate (ivan.theate{at}clin.ucl.ac.be)
        1. UCL, Saint-Luc, Belgium
          1. Patrick Durez (patrick.durez{at}ruma.ucl.ac.be)
          1. UCL, St Luc, Belgium
            1. René Westhovens (rene.westhovens{at}uz.kuleuven.ac.be)
            1. University Hospital Leuven, Belgium
              1. Frank P Luyten (frank.luyten{at}uz.kuleuven.ac.be)
              1. University Hospitals KULeuven, Belgium

                Abstract

                Objective: To characterize the bone morphogenetic protein (BMP) target cells positive for phosphorylated (P-)SMAD1/5, in rheumatoid arthritis (RA) synovium.

                Methods: Synovial biopsies were obtained by needle arthroscopy. Anti-P-SMAD1/5 antibodies were used for Western blot (WB) on protein extracts from RA and normal synovium and for immunostaining of synovial biopsy sections. Positive cells were further identified by double staining for CD3, CD20, CD68, CD138, CD90, alpha smooth muscle actin (SMA), endoglin (CD105) and von Willebrand factor (VWF). In sections from early RA patients taken before and under antirheumatic treatment, the degree of inflammation and activation of the BMP pathway were quantified.

                Results: P-SMAD1/5 protein was detected by WB in RA and to a lesser extent in normal synovium. Different P-SMAD1/5 positive cell populations were identified in RA synovium, mainly in perivascular and sublining cells. P-SMAD1/5 positive perivascular cells were alpha SMA positive and located around VWF positive endothelial cells. Some CD90 positive synovial fibroblasts were P-SMAD1/5 positive, as was part of the CD68 positive synovial cells but other cells of the haematopoietic lineage showed no SMAD1/5 phosphorylation. Treatment resulted in an absolute but not relative decrease in BMP activation in the synovium.

                Conclusion: BMP-activated cells belong to distinct stromal compartments in RA synovium and some of them express markers associated with the mesenchymal progenitor cell lineage. Antirheumatic treatment effectively downregulates synovial inflammation, but BMP activation in the synovium does persist albeit reduced.

                • bone morphogenetic proteins
                • mesenchymal cells
                • rheumatoid arthritis
                • synovium

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