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COMP – a candidate molecule in the pathogenesis of systemic sclerosis with a potential as a disease marker
  1. Roger Hesselstrand (roger.hesselstrand{at}skane.se)
  1. Department of Clinical Sciences, Section for Rheumatology, Lund University, Sweden
    1. Anja Kassner (anja.kassner{at}med.lu.se)
    1. Department of Experimental Medical Sciences, Section for Connective Tissue Biology, Lund University, Sweden
      1. Dick Heinegard (dick.heinegard{at}med.lu.se)
      1. Department of Experimental Medical Sciences, Section for Connective Tissue Biology, Lund University, Sweden
        1. Tore Saxne (tore.saxne{at}med.lu.se)
        1. Department of Clinical Sciences, Section for Rheumatology, Lund University, Sweden

          Abstract

          Objective: COMP primarily found in cartilage is thought to be an important regulator of assembly and maintenance of the fibrillar collagen I and II networks. Recently COMP was shown to be produced by skin fibroblasts from patients with systemic sclerosis (SSc, scleroderma). The purpose of this study was to examine whether COMP is released from skin to serum in SSc patients, and may serve as indicator of activity of skin involvement.

          Methods: Serum COMP levels were measured by enzyme linked immunosorbent assay in patients with SSc whose skin involvement was assessed with the modified Rodnan skin score (mRss) and high frequency ultrasound. The presence of COMP in skin biopsies was assessed by Western blot using a monoclonal antibody to the very C-terminal end of human COMP.

          Results: Serum COMP correlated to skin involvement as measured by the mRss (n=70; rS=0.60; p<0.001), to skin thickness measured by ultrasound (n=88; rS=0.55; p<0.001) and inversely to skin echogenicity measured by ultrasound (n=88; rS=-0.40; p<0.001). In 70 patients followed longitudinally there was a correlation between changes in serum COMP (n=307) and changes in mRss (rS=0.35; p=0.008). In individual patients monitored with repeated measurements, serum COMP changes closely paralleled changes in mRss. A C-terminal COMP fragment, with an apparent Mr 56 kDa, was identified in SSc skin biopsies, while no COMP-reactivity was detected in normal skin.

          Conclusion: The high turnover of COMP in SSc skin suggests a pathophysiologic role. Serum COMP shows promise as a new biomarker in SSc.

          • COMP
          • biomarker
          • scleroderma
          • skin
          • systemic sclerosis

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