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Infliximab inhibits bone resorption by circulating osteoclast precursor cells in patients with Rheumatoid Arthritis and Ankylosing Spondylitis
  1. Michael Gengenbacher
  1. Dept of Rheumatology and Clinical Immunology/Allergology, University Hospital, Inselspital Bern, Switzerland
    1. Hans-Jörg Sebald
    1. Department of Orthopedic Surgery, University Hospital, Inselspital Bern, Switzerland
      1. Peter M Villiger
      1. Dept of Rheumatology and Clinical Immunology/Allergology, University Hospital, Inselspital Bern, Switzerland
        1. Willy Hofstetter
        1. Group of Bone Bilogy, Department of Clinical Research, University of Bern, Switzerland
          1. Michael Seitz (michael.seitz{at}insel.ch)
          1. Dept of Rheumatology and Clinical Immunology/Allergology, University Hospital, Inselspital Bern, Switzerland

            Abstract

            Objective: To examine the effects of infliximab on bone resorption by osteoclast precursor cells (OCPs) in patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS) and to compare the results with changes in disease activity.

            Methods: Before and during 24 weeks of infliximab treatment peripheral blood mononuclear cells of 9 RA and 10 AS patients were seeded onto ivory wafers and adherent cells, including OCPs, were grown in medium promoting osteoclast differentiation. Bone resorption was evaluated morphometrically and correlated to disease activity. 19 healthy individuals were studied in parallel. In addition, biochemical bone markers were assessed in all patients at baseline and after 24 weeks.

            Results: OCPs from RA patients showed a higher bone resorption at baseline when compared to AS patients. Blocking of TNFα with infliximab resulted in a strong reduction of bone resorption by OCPs in both cohorts and did occur faster in RA compared to AS patients. This inhibition coincided with reduction of clinical disease activity in both patient cohorts and with an increase of serum osteocalcin levels and a relative decrease of collagen crosslinks in RA compared to AS patients.

            Conclusion: These results provide an explanation on the cellular level for the anticatabolic effect of TNF neutralization on bone. The variation in the kinetics of bone resorption by the OCPs in patients with RA and AS suggests disease-specific differences in the type or in the preactivation of OCPs.

            • ankylosing spondylitis
            • anti-TNFalpha treatment
            • osteoclast precursor cells
            • rheumatoid arthritis

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