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Phenotypic and functional characterization of ovine mesenchymal stem cells: application to a cartilage defect model
  1. D Mrugala (mrugala{at}montp.inserm.fr)
  1. Inserm U844, France
    1. C Bony (bony{at}montp.inserm.fr)
    1. Inserm U844, France
      1. N Neves (nuno{at}dep.uminho.pt)
      1. 3B's Research Group, Portugal
        1. L Caillot (lcaillot{at}hotmail.com)
        1. Abcys, France
          1. S Fabre (sylviefabre{at}mageos.com)
          1. Lapeyronie Hospital, France
            1. D Moukoko (dsmoukoko{at}aol.com)
            1. Lapeyronie Hospital, France
              1. C Jorgensen (jorgens{at}montp.inserm.fr)
              1. Inserm U844, France
                1. D Noël (noel{at}montp.inserm.fr)
                1. Inserm U844, France

                  Abstract

                  Background: Multipotent mesenchymal stromal cells (MSCs) are of particular interest for their potential clinical use in cartilage engineering but a consistent model is missing in large animals.<p< Objective: In absence of any detailed study reporting a complete characterization of the mesenchymal cells isolated from sheep bone marrow, we fully characterized the adherent stromal cells and developed a pre-clinical model of cartilage engineering by implantation of autologous MSC in the Merinos sheep.

                  Methods: oMSC were isolated from bone marrow, expanded and further characterized according to the recently proposed definition of the MSC. The experimental model consists in partial-thickness lesions created in the inner part of the patellae of the posterior legs. Lesions were filled with oMSC ± chitosan ± TGFβ-3 in a fibrin clot.

                  Results: Ovine MSC (oMSC) were shown to display the three main characteristics of MSC: adherence to plastic, phenotypic profile (positive for CD44, CD105, vimentin and negative for CD34 and CD45) and trilineage differentiation potential. We also report two other important functional characteristics of MSC: support of long term hematopoiesis and immunosuppressive capacity. In vivo, two months after implantation, the histological analysis revealed chondrocyte-like cells surrounded by a hyaline-like cartilaginous matrix that was integrated to the host cartilage when oMSC were combined with chitosan and TGFβ-3.

                  Conclusions: This study provides for the first time a strong characterization of oMSC and establishes the basis for a model of cartilage engineering in a large animal.

                  • cartilage repair
                  • differentiation
                  • haematopoiesis support
                  • immunosuppression
                  • multipotent stromal stem cell

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