Article Text

other Versions

PDF
Differential expression of syndecans and glypicans in chronically inflamed synovium
  1. Angela M Patterson
  1. Arthritis Research Centre, Medical School, Keele University, RJ A H Orthopaedic Hospital, Oswestry, United Kingdom
    1. Alison Cartwright
    1. Arthritis Research Centre, Medical School, Keele University, RJ A H Orthopaedic Hospital, Oswestry, United Kingdom
      1. Guido David
      1. Center for Human Genetics, University of Leuven, Belgium
        1. Oliver Fitzgerald
        1. University Department of Rheumatology, St Vincents Hospital, Dublin, United Kingdom
          1. Barry Bresnihan
          1. University Department of Rheumatology, St Vincents Hospital, Dublin, United Kingdom
            1. Brian A Ashton
            1. Arthritis Research Centre, Medical School, Keele University, RJ A H Orthopaedic Hospital, Oswestry, United Kingdom
              1. Jim FS Middleton (jim.middleton{at}rjah.nhs.uk)
              1. Arthritis Research Centre, Medical School, Keele University, RJ A H Orthopaedic Hospital, Oswestry, United Kingdom

                Abstract

                Background: Membrane-bound heparan sulphate proteoglycans (HSPGs) act as coreceptors and presenters of cytokines and are involved in cell-matrix and cell-cell adhesion.

                Objective: To investigated which HSPGs are expressed in knee joint synovia from patients with different forms of arthritis and normal individuals. Methods: Synovial samples were obtained from patients with early rheumatoid arthritis (n=8), longstanding rheumatoid arthritis (n=13), psoriatic arthritis (n=7), osteoarthritis (n=6) and normal joints (n=12). Expression of syndecan-1, -2, -3 and -4 and glypican-1, -3 and -4 was analysed by immunohistochemistry and dual label immunofluorescence.

                Results: The expression of HSPGs in chronically inflamed synovium exhibited a differential distribution. Syndecan-1 was present in the mononuclear infiltrates of synovia from patients with rheumatoid and psoriatic arthritis where it was expressed by plasma cells. Syndecan-2 was present mainly in blood vessels where it occurred on endothelial cells, pericytes and smooth muscle cells. Syndecan-3 stained intensely in endothelial cells but also occurred in sublining macrophages and the lining layer. Glypican-4 occurred in the lining layer and blood vessels. Increased expression of these HSPGs was apparent in rheumatoid and psoriatic compared to osteoarthritic and normal synovia. Little or no staining for syndecan-4, glypican-1 and glypican-3 was demonstrated in all samples.

                Discussion: Selected HSPGs, such as syndecan-1, -2 and -3 and glypican-4, could play a part in the pathophysiology of arthritis, such as the migration and retention of leukocytes and angiogenesis in the chronically inflamed synovium.

                • expression
                • glypican
                • inflamed
                • syndecan
                • synovium

                Statistics from Altmetric.com

                Request permissions

                If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.