Article Text
Abstract
Objective: To examine the relationship between SNP +39604 in SEEK1 and psoriatic arthritis (PsA) in two distinct Canadian populations.
Methods: 103 patients with PsA and 105 ethnically matched controls from Newfoundland and 202 patients with PsA and 100 controls from Ontario were studied. Patients and controls were genotyped for SNP +39604 of SEEK1 by time of flight mass spectrometry, using the Sequenom platform. Genomic DNA was amplified by the Dynal RELI SSO HLA- Cw* typing kit for HLA-C typing.
Results: The frequency of the minor SEEK1(T) allele in subjects with PsA and controls was 48.5% and 32.4%, respectively (odds ratio (OR) = 2.0; p = 0.017), in the Newfoundland population and 46.5% and 38.0%, respectively (OR = 1.4; p = 0.16), in the Ontario population. Although SEEK1 is associated with PsA, particularly in the Newfoundland population, multivariate analysis showed that SEEK1 does not seem to be a further susceptibility factor if the HLA-Cw*0602 status is already known. No association was noted between SEEK1(T) allele and onset of psoriasis, PsA, or arthritis pattern.
Conclusion:SEEK1 is associated with PsA in the Newfoundland founder population. This association is probably due to linkage disequlibrium between SEEK1 and HLA-Cw*0602 in this population.
- OR. odds ratio,
- PCR, polymerase chain reaction
- PsA, psoriatic arthritis
- SNP, single nucleotide polymorphism
- SEEK1
- founder population
- genetics
- psoriatic arthritis
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Footnotes
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Published Online First 11 February 2005