Background Ankylosing Spondylitis (AS) affects human health seriously, which is difficult to diagnose in the early stages. It is reported that MicroRNAs (miRNAs) may serve as novel biomarkers for AS. Exosome can function as vehicles to deliver miRNAs in body fluids including saliva and plasma. Our previous study shows that exosomal miRNA21–5P is higher expressed in AS patients, compared with healthy subjects. However, the relationship between exosomal miRNA21–5P and AS has yet to be determined.
Objectives The aim of the present study is to explore the relationship between exosomal miRNA21–5P and AS.
Methods AS patients who fulfilled the modified New York criteria were enrolled for this study. Healthy subjects were also enrolled as control group. BASDAI, BASFI, C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were evaluated. Quantitative reverse-transcription PCR (qRT-PCR) was used to confirm the expression of exosomal miRNA21-5P, and receiver-operator characteristic (ROC) curve was used to evaluate the diagnostic value of exosomal miRNA21-5P for AS. According to the cut off value, AS patients were divided into exosomal miRNA21–5P low value group (<cut off value) and exosomal miRNA21-5P high value group (≥cut off value), and the difference of AS patient's clinical characteristics between the two group were explored.
Results Twenty healthy subjects and 38 AS patients were enrolled in the study. The qRT-PCR results indicated that the expression level of exsomal miRNA21-5P in AS patients was (2.041±0.975) times higher than that of healthy subjects. ROC curve analysis showed that exsomal miRNA21-5P had significant diagnostic value for AS with the AUC of 0.809 (CI95%: 0.691–0.921). In addition, the cut off value was 1.310, with the specifity of 80.0% and sensitivity of 76.32%. According to the cut off value of exsomal miRNA21–5P, AS patients were divided into the low exsomal miRNA21-5P group (<1.310) and the high exsomal miRNA21-5P group (≥1.310). Among the 38 AS patients, 12 cases were in the low exsomal miRNA21–5P group and 26 cases were in the high exsomal miRNA21-5P group. Comparations of the clinical characteristics of the two groups showed that BASDAI, BASFI, CRP and ESR were significantly increased in exsomal miRNA21-5P high value group.
Conclusions Exsomal miRNA21-5P was significantly increased in AS patients, which may be used as a biomarker for AS.
Atayde VD, Aslan H, Townsend S, Hassani K, Kamhawi S, Olivier M. Exosome.
Secretion by the Parasitic Protozoan Leishmania within the Sand Fly Midgut. Cell Reports, 2015, 13 (5): 957–67.
Disclosure of Interest None declared