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SAT0157 Mycobacterium tuberculosis specific responses from CD8+ and CD4+ T cells in patients with latent and active form of tuberculosis
  1. E Zanova1,
  2. D Kozakova1,
  3. M Polanova2,
  4. I Solovic2,
  5. I Rybar3
  1. 1National Institute of Rheumatic Diseases, Piešťany
  2. 2National Institute of Tuberculosis, Pulmonary Diseases and Thorax Surgery, Vysne Hagy
  3. 3Slovak Health University, Bratislava, Slovakia

Abstract

Background Testing the presence of latent or active tuberculosis using IGRA tests is necessary part of the diagnostic screening at risk individuals. QuantiFERON-TB Gold Plus (QFT-Plus) is a test for cell-mediated immune (CMI) responses to peptide antigens that simulate mycobacterial proteins. Peptide antigens in TB1 tube elicit CMI responses from CD4+ T-helper lymphocytes, in TB2 tube from CD8+ T-cytotoxic lymphocytes. Test is based on the ability of effector T lymphocytes to produce the cytokine interferon gamma (IFN-γ).

Objectives To compare and evaluate levels of IFN-γ produced by CD4+ and CD8+ cells in TB1 and TB2 tubes.

Methods It was studied 33 subjects with latent form of tuberculosis (LTBI) and 33 subjects with active TB. QFT-Plus was used to detect in vitro responses to peptide antigens associated with Mycobacterium tuberculosis infection (ELISA, QIAGEN). Results were obtained by calculation of INF-γ levels in Mitogen, TB antigen (TB1, TB2) and Negative control tubes using QFT analysis software.

Results QFT-Plus positive results were observed in both groups of people. Increased levels of IFN-γ produced by CD4+ cells (TB1) has been detected in 21 subjects with LTBI (63,64%) and in all 33 subjects with active TB (100,0%). Increased levels of IFN-γ produced by CD8+ cells (TB2) has been detected in 27 people with LTBI (81,82%) and in all 33 people with active TB (100,0%). Joint increase levels of IFN-γ in the tubes TB1 and TB2 was observed in 16 individuals with LTBI (48,48%) and in all 33 individuals with active TB (100,0%). In the group of patients with a negative QFT-Plus it was not observed increased levels of IFN in tubes TB1 and TB2.

Conclusions Our findings confirm specific CD4+ and CD8+ T cell response to mycobacterial protein antigens in individuals with LTBI and also in active TB subjects, in which INF-γ was frequently found. The immunological response represented by CD4+ and CD8+ T cells was not detected in subjects with QFT-Plus negative.

Disclosure of Interest None declared

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