Objectives The objective is to study association of genotypes for Bcl1 polymorphism in the glucocorticoid receptor (GR) gene with ischemic heart disease (IHD) in patients with rheumatoid arthritis (RA).
Methods 161 subjects with rheumatoid arthritis aged 40 years and older were examined by means of instrumental, clinical and laboratory examinations. Rheumatoid arthritis was diagnosed according to ACR/EULAR Classification Criteria (2010). The IHD diagnosis was verified by means of AHA/ACC guidelines (2012). BCL1 polymorphism in exon 2 was identified using polymerase chain reaction with subsequent analysis of restriction fragment length polymorphism by Fleury I. et al. (venous blood was used as the material for the study). Statistical analysis was performed using SPSS–17 program.
Results It was revealed that 76 (47.2%) patients had isolated RA (group I), while 85 (52.8%) individuals had RA with concomitant IHD (group II). In group I, there were 29 (38.2%) patients with C/C genotype, 39 (51.3%) – with C/G genotype, and 8 (10.5%) – with G/G genotype. The distribution in group I was as following: 16 were homozygous for the C allele (18.8%), 40 were heterozygous (47.1%) and 29 were homozygous for the G allele (34.1%) (χ2=15.23; p=0.02). It was established that the risk of ischemic heart disease was 6.57 times higher in homozygotes for the G allele (OR=6.57; 95% CI=2.44−17.73; p=0.001) as compared with homozygotes for the C allele.
Conclusions It was established that G/G genotype prevailed in RA patients with ischemic heart disease, while C/C genotype prevailed in patients with isolated RA. The risk of IHD development in patients with RA was associated with G/G genotype for Bcl1 polymorphism in the GR gene.
Disclosure of Interest None declared