Article Text

PDF
SAT0024 Alterations of peripheral blood B-cell subsets in early rheumatoid arthritis
  1. E Suponitskaya,
  2. A Avdeeva,
  3. AA Aleksankin,
  4. E Gerasimova,
  5. E Aleksandrova,
  6. T Popkova,
  7. AN Novikov,
  8. DK Karateev
  1. Institute of Rheumatology, Moscow, Russian Federation

Abstract

Background Alterations of B-cell subpopulation have been described in the peripheral blood of rheumatoid arthritis (RA) patients (pts), but differences between B-cell subsets distributions in early and late onset RA (LORA) are still unclear [1,2].

Objectives To examine B-cell subsets in peripheral blood of healthy donors, early (disease duration <6 months) RA (ERA) and LORA pts by flow cytometry, and to analyze the association between B-cell subsets and RA activity.

Methods 24 active ERA pts (20F/4M); median age 54 years (range) (38–64); disease duration 5 months (4–6); DAS28 score 5.2 (4.7–5.9); RF+75%, ACCP+ 87.5% and 20 LORA pts (16F/4M); median age 58 years (range) (44–62); disease duration 12 years (4.5–17.5); DAS28 score 5.3 (4.6–6.2); both RF+ and ACCP+ 80%, were assessed for B-cell subpopulations and laboratory data: ESR, CRP. LORA pts were treated with DMARDs (anti-TNF-α, Methotrexate), glucocorticoids, and NSAIDS; ERA pts received NSAIDS only. CD19+B cells, memory B cells (CD19+CD27-), non-switched memory B cells (CD19+IgD+CD27+), switched memory B cells (CD19+IgD-CD27+), naïve (CD19+IgD+CD27-), double-negative (CD19+IgD-CD27-), transitional (CD19+IgD+CD10+CD38++CD27-) B cells, and plasmablasts (CD19+CD38+++IgD-CD27+CD20-) were analyzed using multicolor flow cytometry.

Results The median percentages (range) of non-switched memory B cells (CD19+IgD+CD27+) were lower in ERA and LORA RA cohorts compared to donors: 3.0% (1.6–5.5) and 6.2% (2.4–10.2) vs 7.4% (3.7–11.1), respectively, p<0.05 for both cases. In LORA pts, the median percentages (range) and absolute numbers of switched memory B cells (CD19+IgD+CD27-) were higher compared to both ERA pts and donors: 24,1% (14,0–42.3) vs 8,3% (4.4–12.9) and 12.8% (9.3–17.0); and 0.03 (0.02–0.05) vs 0.01 (0.006–0.02) and 0.02 (0.01–0.04), respectively; p<0.05 for all cases. The median percentages of naïve B cells (CD19+IgD+CD27-) were significantly higher in ERA pts compared to LORA pts and healthy donors: 75.3% (69.7–83.6) vs 54.3% (39.7–69.1) and 64.7% (57.6–72.4), respectively, p<0.05 for both cases.

In ERA pts, we found a significant correlation between absolute numbers of non-switched memory B cells (CD19+IgD+CD27+), RF and anti-CCP: r=0.50 and 0.45, respectively, p<0.05 for both cases. In LORA pts, the percentage of memory B cells (CD19+CD27+) correlated positively with ESR (r=0.58), p<0.05; a positive association was observed between the absolute numbers of switched memory B cells (CD19+IgD-CD27+), CRP (r=0.48) and RF (r=0.49), p<0.05 for all cases.

Conclusions in ERA pts, immunophenotyping showed increased frequencies of naïve B cells and decreased frequencies and absolute numbers of switched memory B cells compared to LORA cohort.

References

  1. Fedele et al. BMC Immunology 2014, 15:28. doi: 10.1186/s12865-014-0028-1. Memory B cell subsets and plasmablasts are lower in early than in long-standing Rheumatoid Arthritis.

  2. Moura RA et al. Rheumatology 2010 Jun;49(6):1082–92. doi: 10.1093/rheumatology/keq029. Alterations on peripheral blood B-cell subpopulations in very early arthritis patients.

References

Disclosure of Interest None declared

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.