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FRI0423 Antibodies to type ii collagen: a novel tool for the spondyloarthritis diagnosis?
  1. C Vinci1,2,
  2. M Infantino3,
  3. P Pozzilli2,
  4. V Grossi3,
  5. M Manfredi3,
  6. F Bandinelli4,
  7. F Li Gobbi4,
  8. A Damiani4,
  9. R Strollo2,
  10. M Benucci4,
  11. A Nissim1
  1. 1Biochemical Pharmacology, Queen Mary University of London, London, United Kingdom
  2. 2Endocrinology and Diabetes, Campus Biomedico, Rome
  3. 3Immunology and Allergology Laboratory Unit
  4. 4Rheumatology Unit, San Giovanni di Dio Hospital, Florence, Italy

Abstract

Background Spondyloarthritis (SpA) are an inflammatory joint disease with chronic, progressive, axial inflammation of the spine and the sacroiliac joints. Diagnosis of SpA is done criteria by clinical symptoms, radiology and MRI or ultrasound following ASAS criteria. AS is similar to rheumatoid arthritis (RA) and psoriatic arthritis (PsA) as they are all inflammatory joint disease. Nevertheless they show considerable different pathology. [1]

Objectives The aim of our study is to test whether a novel assay that we developed for RA can be used for SpA diagnosis. We have previously showed that antibodies to oxidative post-translationally modified collagen type II (oxPTM-CII) are present specifically in RA patients whether ACPA positive or negative. [2]

Our study intends to investigate the reactivity to oxPTM-CII in SpA patients in comparison to early undifferentiated arthritis (EUA) and PsA patients.

Methods oxPTM-CII were generated using ribose and various reactive oxidants, and then they were analysed by SDS-PAGE. Binding to native and oxPTM-CII was evaluated by ELISA and Western Blotting. We used a cohort of sera from 67 patients with SpA, 54 patients with PsA, 49 patients with EUA. As control we used 19 patients with fibromyalgia (FM) and 70 healthy subjects. The specificity of the binding was further assessed by competitive ELISA and western blot.

Results We detected stronger reactivity to SpA compared to PsA and even EUA serum samples. Hence specific binding to oxPTM-CII was seen in the 52% of SpA sera compared to 12% in PsA and 10% in EUA. There was no binding in samples from FM and healthy individuals. A group of the most reactive SpA samples was evaluated by western blot confirming a strong binding to several fragments or aggregates of oxPTM-CII.

Conclusions For the first time we demonstrated that anti-ROS-CII may become a biomarker for SpA diagnosis.

References

  1. J. Braun and J. Sieper, Ankylosing spondylitis. Lancet, vol. 369, no. 9570, pp. 1379–1390, 2007.

  2. R. Strollo, F. Ponchel, V. Malmström, P. Rizzo, M. Bombardieri, C. Y. Wenham, R. Landy, D. Perret, F. Watt, V. M. Corrigall, P. G. Winyard, P. Pozzilli, P. G. Conaghan, G. S. Panayi, L. Klareskog, P. Emery, and A. Nissim, Autoantibodies to posttranslationally modified type II collagen as potential biomarkers for rheumatoid arthritis. Arthritis Rheum., vol. 65, no. 7, pp. 1702–12, Jul. 2013.

References

Disclosure of Interest None declared

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