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FRI0403 Increased crp level is interrelated with clinical and serological phenotypes of systemic scleroderma and associated with crp rs1205 gene polymorphism
  1. M Krylov1,
  2. L Ananieva1,
  3. O Koneva1,
  4. M Starovoytova1,
  5. O Desinova1,
  6. O Ovsaynnikova1,
  7. E Aleksandrova1,
  8. A Novikov1,
  9. I Guseva1,
  10. N Konovalova2,
  11. D Varlamov2
  1. 1V. A. Nasonova Research Institute of Rheumatology
  2. 2All-Russian Research Institute of Agricultural Biotechnology, Moscow, Russian Federation

Abstract

Background Recent studies report on increased serum levels of C-reactive protein (CRP) in systemic scleroderma (SSc) pts.

Objectives The current study was aimed at identifying potential correlations between CRP levels, immunologic SSc phenotypes and CRP rs1205 gene polymorphism in the Russian cohort.

Methods 92 SSc pts were enrolled in the study. Mean age (± SD) was 49,4±12,6 years, mean (± SD) SSc duration was 11,1±9,0 years, increased CRP levels >5 mg/l were documented in 43% pts. CRP levels were analyzed in the following subgroups: with limited (lcSSc) and diffuse (dcSSC) types, with SSc duration <3 years >3, with or without interstitial lung disease (ILD+) and (ILD-), as well as in subgroups with positive antibody titers to DNA topoisomerase I (ATA+) and antibody to centromeres (ACA+). CRP concentrations were measured with highly sensitive immunoturbidimertry method. Allele-specific real time PCR was used to study CRP rs1205 gene polymorphism.

Results Mean CRP (± SD) level was significantly higher in dcSSc vs lcSSc (11,9±15,5 mg/l; vs 4,7±7,4 mg/l, respectively, p=0,006). Pts with disease duration <3 years had higher mean CRP levels as compared to pts with SSc duration >3 years, although the difference was not significant. In pts with disease duration >3 years mean CRP levels were significantly higher among subjects with dcSSc than lcSSc (11,0±15,5 mg/l vs 4,8±7,7 mg/l, respectively, p=0,027). Mean CRP level was higher in (ATA+) pts than in (ATA-) pts (11,0±15,4 mg/l vs 5,5±13,7 mg/l, respectively, p=0,032). No statistical difference in CRP levels was found between (ILD+) and (ILD-) pts, as well as between (ACA+) and (ACA-) pts.

Carriers of rs1205 CC genotype had higher CRP levels than carriers of rs1205 T allele,p=0,087). In pts with SSc duration >3 years and carriers of rs1205 CC genotype mean CRP level was significantly higher than in T-allele carriers (12,0±17,4 mg/l vs 5,4±7,4 mg/l, respectively, p=0,021). Similar trends for CRP levels depending on genotypes were established among (ATA+) pts (16,9±20,1 mg/l and 7,3±10,5 mg/l, respectively, p=0,031).

Conclusions Increased CRP levels were found almost in 50% of Russian SSc pts. Higher CRP levels were associated with specific clinical and immunological SSc parameters and CRP rs1205 CC genotype, signifying unfavorable prognosis.

Disclosure of Interest None declared

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