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FRI0051 Early arthritis induces disturbances at bone nanostructural level reflected in decreased tissue hardness
  1. B Vidal1,
  2. R Cascão1,
  3. M Finnilä2,3,
  4. I Lopes1,
  5. S Saarakkala2,4,5,
  6. P Zioupos6,
  7. H Canhão7,
  8. J Fonseca1,8
  1. 1Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal
  2. 2Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu, Oulo
  3. 3Department of Applied Physics, University of Eastern Finland, Kuopio
  4. 4Medical Research Center Oulo, Oulu University
  5. 5Department of Diagnostic Radiology, Oulu University Hospital, Oulo, Finland
  6. 6Biomechanics Labs, Cranfield Forensic Institute, Cranfield University, Swindon, United Kingdom
  7. 7EpiDoC Unit, CEDOC, NOVA Medical School, NOVA University
  8. 8Rheumatology Department, Centro Hospitalar de Lisboa Norte, EPE, Hospital de Santa Maria, Lisbon Academic Medical Centre, Lisboa, Portugal

Abstract

Background Arthritis induces joint erosions and skeletal bone fragility.

Objectives The main goal of this work was to analyze the early arthritis induced events at bone tissue level.

Methods Eighty-eight Wistar rats were randomly housed in experimental groups, as follows: adjuvant induced arthritis (N=47) and a control healthy group (N=41). Rats were monitored during 22 days for the inflammatory score, ankle perimeter and body weight and sacrificed at different time points (11 and 22 days post disease induction). Bone samples were collected for histology, micro-CT, 3-point bending, nanoindentation and Fourier transformed infrared spectroscopy (FTIR) analysis. Blood samples were also collected for bone turnover markers and systemic cytokine quantification.

Results At bone tissue level, measured by FTIR analysis and nanoindentation, there was a reduction of the mineral and collagen content and of hardness in the arthritic group, associated with an increase of the ratio of bone concentric to parallel lamellae and of the area of the osteocyte lacuna. In addition, increased bone turnover and changes in the microstructure and mechanical properties were observed in arthritic animals, since the early phase of arthritis, when compared with healthy controls.

Conclusions Arthritis induces very early changes at bone tissue level characterized by decreased tissue hardness and of collagen and mineral content. These observations highlight the pertinence of immediate control of inflammation in the initial stages of arthritis.

Disclosure of Interest None declared

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