Background Rheumatoid arthritis (RA) is an autoimunme disease.The DMARDs is mian treatment but side effects are very high. Bone marrow mesenchymal stem cells (MSCs) have immunoregulatory function so it can be used to treat RA. This study was designed to investigate the effect of BMSCs on chemokines in CIA rats.
Objectives To observe the immunomodulation effects of BM-MSCs on the Inflammatory Chemokines of rats with collagen-induced arthritis (CIA).
Methods Rats MSCs were isolated and expanded from bone marrow cells by density gradient centrifugation and adhering to the culture plastic bottle, and the phenotypes were assessed by flow cytometry. We established collagen induced arthritis rats model. A total of six groups. The MSCs treatment groups contain the early and advanced stages. Meanwhile, there are normal control and CIA control group,The latter also contains the early and advanced stages. Also,there is the positive control group of MTX, by intraperitoneal injection of a small dose of MTX (0.9mg/kg/w), for six weeks. 1x106 /kg BM-MSCs were implanted through tail vein to MSCs treatment groups, model control groups with equal amounts of saline served as controls.At forty two day,stream multi-factor detection technique were used to detect the level of RANTES, MCP-1 and IP-10 in serum of CIA rats.
Results The level of RANTES, AMCP-1, AIP-10 was higher in CIA control group than normal group (P<0.05); After MTX treatment, these factors level were significantly difference than the CIA control group (P<0.05). These factors level were lower in early BMSCs treatment groups than early CIA control groups (P<0.05), while the late MSCs treatment group versus the later CIA control group showed no statistically significant difference (P>0.05). The early and late MSCs treatment group versus the MTX treatment group showed no statistically significant difference (P>0.05).
Conclusions In this study MSCs has shown significant immune modulatory effects. It down regulates the level of Inflammatory Chemokines in CIA rats. The early treatment group is more effective than the late treatment group.
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Acknowledgements Mesenchymal stem cell;Arthritis,rheumatoid; Collagen II induced arthritis (CIA);Inflammatory chemokine.
Disclosure of Interest None declared