Background Osteoarthritis (OA), is an age-related common polygenic disease characterized by the thinning and loss of the articular cartilage in synovial joints such as knees. The etiology and pathogenesis of OA is largely unknown, the single-nucleotide polymorphism (SNP) rs143383 (C/T) influencing OA susceptibility across a range of ethnic groups.
Objectives The present study investigated to identify the association of polymorphism in GDF-5 gene with osteoarthritis in Egyptian population.
Methods This Case control study of 100 women and 100 male ≥40 years that fulfilled American College of Rheumatology (ACR) for Knee OA and 100 controls recruited from the outpatient clinic of Department of Rheumatology, Asyut university, Egypt. Clinical symptoms were assessed with WOMAC index and VAS for knee pain. The severity of disease was determined by radiological grades (Kellgren Lawren). Body Mass Index (BMI) was recorded. DNA isolation and genotype analysis the method of (Southam et al., 2007) was followed for determining the GDF-5 gene (T/C; rs143383) polymorphism. Amplification was performed.
Results There were weak but significant associations present between the GDF5 polymorphism and knee OA at the allele level (C vs. T: 0.85, 95% CI =0.78–0.93) and genotype level (CC vs. TT: 0.72; CT vs. TT: 0.81; CC/CT vs. TT: 0.83; CC vs. CT/TT: 0.78) in the overall population. A stronger significant association was observed for CC vs. TT (OR =0.83, P<0.001) in comparison with other models. In males we identified a second polymorphism, located in the 3'-UTR of GDF5, that influenced allelic expression of the gene independent of rs143383.
Conclusions GDF5 is an OA susceptibility gene with association between the GDF5 polymorphism and clinical symptoms of knee OA in Egyptian population.
Disclosure of Interest None declared