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AB0242 Evaluation of variants in MIR-146A, MIR-196A-2 and MIR-499 and their association with susceptibility for rheumatoid arthritis and its extra-articular manifestations
  1. JC De La Cruz-Castillejos,
  2. RE Barbosa-Cobos,
  3. LT Becerril-Mendoza,
  4. GE Lugo-Zamudio,
  5. J Ramírez-Bello,
  6. M Matias-Carmona,
  7. I Alemán-Άvila
  1. Hospital Juárez de México, Ciudad de México, Mexico

Abstract

Background The miRNAs, small non-coding RNA, regulate the genetic expression to posttranscriptional level, inhibiting the translation. The role of miRNAs in the evolution of RA is not clear.

Objectives To evaluate the variants rs 2910164G/C in miR-146a, rs11614913C/T in miR-196a-2 and rs3746444A/G in miR-499 and their association with susceptibility and severiry to Rheumatoid arthritis (RA) and its extra-articular manifestations (EAM)

Methods 133 cases with RA were included (ACR/EULAR criteria 2010) and 430 healthy controls. There were evaluated EAM (rheumatoid nodules [RN], Raynaud phenomenon [RP], cutaneous vasculitis (CV), episcleritis, scleritis, peripheral ulcerative keratitis [PUK], multiple mononeuritis [MM] and multiple polyneuritis [MP]) and levels of ESR, CRP, RF and CCP. It was performed genotyping of singe nucleotide polymorphisms (SNPs) rs2910164G/C in miR-146a, rs11614913C/T of miR-196a-2 and rs3746444A/G of miR-499. The descriptive and inferential statistical analysis was performed with the software SPSS and Finetti.

Results Patients with RA, women 126 (94.7%); age Me 48.9 (IQR 40–58); patients with EAM 23 (17.2%; women 22 [95.6%]; RN 14 [60.8%], RP 4 [17.3%], CV 1 [4.3%], episcleritis 1 [4.3%], PUK 1 [4.3%], MM 1 [4.3%], MP 1 [4.3%]); ESR Me 37 (IQR 22–45), CRP Me 0.11 (IQR 0.03–0.27); positive RF 125 patients (93.9%, high positive 106 [79.7%], low positive 19 [14.3%]; EAM with high positive RF, 100%), positive CCP 70 (52.6%, high positive 48.9%, low positive 3.8%; EAM high positive 94.1%, negative 5.9%). The alleles and genotypic frequencies did not show statistically significant difference between cases and the healthy controls (p>0.05). It was identified statistical difference between the patients with and without EAM in CPR (p=0.032). The genotypic and allelic frequencies and association analysis of miRNAs in patients with and without EAM are shown in table 1

Conclusions None of the evaluated variants in miRNAs are associated with susceptibility for RA, however, the SNP re11614913C/T licated in miR-196a-2 is associated with EAM.

References

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References

Disclosure of Interest None declared

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