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OP0136 Microrna-223-3p expression in affected skin of adult iga vasculitis correlates with the severity of skin involvement
  1. A Hocevar1,
  2. J Pizem2,
  3. M Tomsic1,
  4. D Glavac2
  1. 1Department of Rheumatology, University Medical Centre Ljubljana
  2. 2Institute of Pathology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia

Abstract

Background IgA vasculitis (IgAV) represents a common systemic vasculitis in paediatric and adult population. Our current knowledge of disease pathogenesis is still very limited and there is no information on miRNAs profile in IgAV.

Objectives The aim of our study was to determine the expression of three miRNAs (miR-148–3p, miR-155–5p and miR-223–3p) in the affected skin of adult IgAV patients.

Methods The study included 65 skin samples from consecutive, untreated IgAV patients (61% male, median age 67.6 years, range 29–91), diagnosed between October 2014 and September 2016, and 10 control skin samples. Total RNA was isolated from tissue section of formalin-fixed, paraffin-embedded samples of biopsied IgAV vasculitic skin lesions and normal skin samples. Expression of miR-148–3p, miR-155–5p and miR-223–3p was measured using qRT-PCR. Skin miRNAs expression was then correlated to clinical characteristics of adult IgAV patients. To present relative miRNA expression the ΔΔCT method was used.

Results We found significantly higher expression levels of miR-223–3p in the affected skin compared to controls (14-fold; p<0.001). The expression of the 148b-3p and miR-155–5p was near normal levels (1.05-fold and 1.13-fold increase, respectively). The differences in the expression of miR-223–3p depending on clinical parameters of IgAV are presented in Table 1. Patients with necrotic skin lesions had significantly higher miR-223 tissue expression than those with non-necrotic purpura (p=0.020). Gastrointestinal tract (GIT) involvement inversely correlated with the level of skin miR-223 expression (p=0.024). No significant relationship between renal involvement and skin miR-223 was found.

Table 1.

miR-223–3p expression in IgAV

Conclusions miR-223 expression was increased in the affected skin of IgAV in comparison to normal skin. Levels of miR-223 expression correlated with severity of skin involvement and inversely with GIT involvement.

Disclosure of Interest None declared

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