Background Immunologic deranging and persistent inflammation is closely associated with the arising and developing of rheumatoid arthritis (RA). Increasing investigators demonstrated microvesicles (MVs) derived from mesenchymal stem cell (MSC-MVs) might simulate immune regulation and tissue repair of the parental cells. However,the immunotherapeutic potential of MVs in RA remains unknown.
Objectives We investigated the therapeutic effects of MSC-MVs in RA model collagen induced arthritis (CIA) rats.
Methods We tested the therapeutic effects of MSC-MVs on CIA rats,levels of T helper 17(Th17), regulatory T cell,and cytokines related,as well as specific transcriptional regulation factor Foxp3 and ROR-γT analysis was performed.
Results Here, we show that MSC-MVs administration effectively improve arthritic symptoms, inhibit synovial hyperplasia,thus delaying the progression to inflammatory bone destruction, as effective as their original cells, exerting arthralprotective effects. MSC-MVs treating inhibited the proliferation of T cell,accelerated the apoptosis. MSC-MVs treating reduce proinflammatory cell Th17,cytokines IL-17, while a incline in the level of an-inflammatory cell Treg,ncytokines TGF-β. In the spllen and ankle joint of CIA rats, MSC-MVs treating increased the expression level of Foxp3 and coinciding with the ROR-γT suppressed, which, the enhanced therapeutic effects correlated with the increase of dosage in a certain range.
Conclusions MSC-MVs showing anti-inflammatory and immunomodulatory activities on CIA rats, suggesting a new and feasible strategy for protection against RA.
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Disclosure of Interest None declared