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OP0132 Low-dose INTERLEUKIN-2 selectively restore regulatory T cell numbers in patients with behcet's disease
  1. X Fu1,
  2. J Li2,
  3. X Li2,
  4. J Luo2,
  5. C Gao2,
  6. Q Chen2
  1. 1Department of Rheumatism
  2. 2The Second Hospital of Shanxi Medical University, Taiyuan, China

Abstract

Background The lack of CD4+CD25+Foxp3+ T regulatory cell (Treg) has been associated with human systemic autoimmune diseases, such as Behcet's disease (BD). IL-2, an essential growth and survival factor for Treg cells.However, the significance of Treg cells in the pathogenesis and the effect of low dose of IL-2 on BD are remain to investigate.

Objectives The lack of CD4+CD25+Foxp3+ T regulatory cell (Treg) has been associated with human systemic autoimmune diseases, such as Behcet's disease (BD). IL-2, an essential growth and survival factor for Treg cells.However, the significance of Treg cells in the pathogenesis and the effect of low dose of IL-2 on BD are remain to investigate.

Methods Eighty patients with BD and seventy healthy donors were enrolled. CD4+T cell subsets in peripheral blood mononuclear cells from these people were measured by multicolour flow cytometry. Twenty-six patients were treated daily with subcutaneous injections of 0.5 million IU of human IL-2 for five consecutive days, CD4+T cell subsets were analysed before and after treatment by flow cytometry.

Results Compared to health control,the absolute counts of circulating Treg cells were significantanty decreased in patients with BD (median:29.93 cell/uL VS median:33.16 cell/uL; p=0.039) and it is negative correlation with disease activity. While the ratios of Th17/Treg in patients with BD (median:0.29;n=80,p=0.034) were significantly higher than those of health control (median:0.2;n=70).No diffrernce in the absolute counts of circulating Th17 cells (CD4+IL-17+) between patients with BD and health control. Treatment of patients with BD with a low-dose of IL-2 regimen selectively increased the absolute counts of Treg cells, from a median of 18.97cell/uL to 74.68 cell/uL (at 5 days) (p=0.000). No significant difference was observed in the absolute counts of circulating Th17, Th1 and Th2 cells after IL-2 treatment.

Conclusions Th17/Treg cells may play a role in the pathogenesis of Patients with BD, low-dose of IL-2 proposes a selective biological treatment strategy by restoring immune tolerance.

Disclosure of Interest None declared

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