Background Rheumatoid arthritis (RA) is a autoimmune disease,which is characterized by the osteoclasia or the high deformity rate of cartilage and bone. According to some materials, Mesenchymal stem cells (MSCs) were definited as the cell full of proliferation, differentiation capacity, and potential immune regulation. MSCs transplantation could be a appropriate-designed pattern to the joint damaging from rheumatoid arthritis (RA).However, the repairing mechanism against osteoclasia of cartilage and bone is still unclear.
Objectives This study used collagen induced arthritis (CIA) rats as animal model to explore MSCs' tissue repairing mechanism.
Methods We observed the ability of BMSCs differentiating into cartilage cells by toluidine blue staining in vitro;Then,BMSCs were labeled by the green fluorescent protein (GFP), infused into CIA through rats' tail vein infusion. In different point time, the rat's joints were made paraffin section,we observed the differentation of GFP positive cells and the distribution of GFP-positive cells differentiated chondrocytes by immunohistochemicale method.
Results First,we found BMSCs in vitro can differentiate into cartilage cells under a certain-culture condition. Then, BMSCs were labeled by the green fluorescent protein (GFP), infused into CIA through rats' tail vein infusion. In different point time, the rat's joints were made paraffin section,which the GFP positive cells were observed in synovium and bone marrow tissues after transplantation on the 3th day, and in cartilage tissues on the 11th day, then increased in cartilage tissues on the 30th day,42th day, by laser scanning confocal microscope. Anti-type II collagen, GFP double positive cells were found in articular cartilages (especially damaged part)by Anti-II collagen immunofluorescence technology.
Conclusions BMSCs were restricted to the joint injury or inflammatory site,differentiated into chondrocytes, and then participated in the cartilage repairing.
Zhao, C., L. Zhang, W. Kong, J. Liang, X. Xu, H. Wu, X. Feng, B. Hua, H. Wang, and L. Sun, Umbilical Cord-Derived Mesenchymal Stem Cells Inhibit Cadherin-11 Expression by Fibroblast-Like Synoviocytes in Rheumatoid Arthritis. J Immunol Res, 2015. 2015: p. 137695.
Arend, W.P., G. Mehta, A.H. Antonioli, M. Takahashi, K. Takahashi, G.L. Stahl, V.M. Holers, and N.K. Banda, Roles of adipocytes and fibroblasts in activation of the alternative pathway of complement in inflammatory arthritis in mice. J Immunol, 2013. 190(12): p. 6423–33.
Takano, T., Y.J. Li, A. Kukita, T. Yamaza, Y. Ayukawa, K. Moriyama, N. Uehara, H. Nomiyama, K. Koyano, and T. Kukita, Mesenchymal stemcells markedly suppress inflammatory bone destruction in rats with adjuvant-induced arthritis. Lab Invest, 2014. 94(3): p. 286–96.
Gupta, A., C.D. Kaur, M. Jangdey, and S. Saraf, Matrix metalloproteinase enzymes and their naturally derived inhibitors: novel targets in photocarcinoma therapy. Ageing Res Rev, 2014. 13: p. 65–74.
Oshita, K., K. Yamaoka and Y. Tanaka, [Regulation of osteoclastogenesis by human mesenchymal stem cells leading to application of a novel treatment for rheumatoid arthritis]. J UOEH, 2013. 35(1): p. 33–7.
Disclosure of Interest None declared