Article Text

PDF
08.08 Podocytes internalise dna-antibody complexes
  1. Anja Römer-Hillmann1,2,
  2. Elisabeth Jung2,3,
  3. Annika Engbers1,
  4. Marcel Reinhardt1,
  5. Hedda Wardemann4,
  6. Thomas Pap1,
  7. Annett Jacobi2,5
  1. 1Institute of Experimental Musculoskeletal Medicine, University Hospital Münster, Germany
  2. 2Department of Internal Medicine D, Nephrology and Rheumatology, University Hospital Münster, Germany
  3. 3Department of Paediatric Haematology and Oncology, University Children´s Hospital Münster, Germany
  4. 4Max Planck Institute for Infection Biology, Berlin, Germany
  5. 5Internal Medicine, Ruppiner Kliniken GmbH, Neuruppin, Germany

Abstract

Background Podocytes are postmitotic visceral epithelial cells, located at the Bowmans capsule of the kidney building up the slit diaphragm with their foot processes to filtrate the blood and keep valuable large proteins in the vessels.

In Systemic Lupus Erythematosus (SLE) different organs are affected by autoantibodies which results in chronic inflammation and a high percentage of patients develop Lupus nephritis (LN) resulting in podocyte damage and disruption of the slit diaphragm. The risk for LN correlates directly with the level of anti-double stranded DNA antibodies (adsDNAabs).

In this study, we wanted to identify the direct effect of adsDNAabs on podocytes.

Methods We established an in vitro system to investigate the specific impact of monoclonal antibodies and their immune complexes directly on podocytes (AB8 cell line). Monoclonal antibodies were generated by transfecting HEK293T cell line with Ig heavy and corresponding light chain encoding plasmid DNA obtained from single B cells of patients with SLE and LN. Podocytes were incubated with the produced monoclonal antibodies and the internalisation process and functional consequences were analysed by immunofluorescence, Western Blot, FACS and electron microscopy. For comparison, primary human podocytes were isolated from LN patient urine and analysed by immunofluorescence.

Results The recombinantly produced adsDNAabs from LN patients were specific against nuclear structures and build complexes with double stranded DNA, essential for the internalisation by cultivated podocytes. Interestingly, viability and migratory capacity were not influenced by the exposure to adsDNAabs for 48 hour. The internalised antibodies were enclosed by membranous structures and reach the cytosol via clathrin dependent endocytosis. The process of internalisation was time and dose dependent and reversible. Furthermore, internalised antibody complexes could also be detected in primary human LN podocytes.

Conclusion In our in vitro model the specific uptake of adsDNAabs was observed, in line with human LN data. Podocytes from patients with LN show IgG positive aggregates in the cytosol. The internalisation depends on the complex formation of the antibodies with dsDNA and could be important part of the LN pathogenesis.

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.