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04.21 Oncostatin m induces inflammation, angiogenesis and notch signalling in the ra joints, and displays pleiotropic effects on tnfα-induced pro-inflammatory effects
  1. Megan Hanlon1,
  2. Doug Veale2,
  3. Tatsiana Rakovich1,
  4. Siobhan Wade1,
  5. Monika Biniecka2,
  6. Ursula Fearon1,
  7. Trudy McGarry1
  1. 1Molecular Rheumatology, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, Republic of Ireland
  2. 2Centre for Arthritis and Rheumatic Diseases, St Vincents University Hospital, University College Dublin, Dublin, Republic of Ireland

Abstract

Background Oncostatin M (OSM) is a pleiotropic cytokine that has both agonistic and antagonistic effects depending on the inflammatory microenvironment. This study examines the effect of OSM on TNFα-induced pro-inflammatory mechanisms and on Notch-1 signalling in Rheumatoid Arthritis.

Methods Primary RA synovial fibroblasts (RASFC) isolated from RA synovial biopsies obtained at time of knee arthroscopy and human dermal microvascular endothelial cells (HMVEC) were grown to confluence. RASFC and HMVEC were cultured with OSM (10ngml) alone or in combination with increasing concentrations of TNFα (0.01–1 ng/ml). IL-6, IL-8, RANTES, GROα and MCP-1 cytokines/chemokines were quantified in culture supernatants by ELISA. Functionally, angiogenesis and invasion were assessed by matrigel tube formation and Transwell invasions assays, VEGF and β1-integrin in cell lysates by Real-time PCR. Finally, Notch-1, its ligands Delta-like-ligand 4 (DLL4) and Jagged-1 (Jag-1) and downstream transcriptional repressors-Hey-1 and Hey-2 were quantified by Real-time PCR.

Results OSM alone significantly induced IL-6 and MCP-1 while inhibiting IL-8 and GROα in RASFC and HMVEC culture supernatants. OSM alone induced RANTES expression in HMVEC with no effect observed for RASFC. OSM potentiated the effect of TNFα on IL-6 and MCP-1 secretion from RASFC and HMVEC. Conversely OSM inhibited TNFα-induced-IL-8 and GROα secretion from RASFC and HMVEC. Interestingly, OSM significantly inhibited TNFα-induced RANTES expression in HMVEC and conversely potentiated this effect in RASFC. At a functional level, OSM induced both RASFC and HMVEC invasion and network formation and induced VEGF expression compared to basal control. Finally OSM significantly induced Notch-1 and Hey-2 in RASFC and HMVEC in a time dependent manner, but interestingly differentially regulated the Notch-1 ligands, with induction of Jag-1 only observed in RASFC, and induction of DLL-4 only observed in HMVEC.

Conclusion OSM is a pleiotropic cytokine that differentially regulates pro-inflammatory mechanisms within the inflamed joint, effects that appear to be dependent on cell type and the inflammatory microenvironment. Targeting OSM or downstream signalling pathways may have therapeutic potential.

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