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Systemic inhibition of IL-6/Stat3 signalling protects against experimental osteoarthritis
  1. Augustin Latourte1,2,
  2. Chahrazad Cherifi1,
  3. Jérémy Maillet1,2,
  4. Hang-Korng Ea1,2,
  5. Wafa Bouaziz1,
  6. Thomas Funck-Brentano1,2,
  7. Martine Cohen-Solal1,2,
  8. Eric Hay1,
  9. Pascal Richette1,2
  1. 1INSERM U1132, Hôpital Lariboisière, Paris, France
  2. 2Rheumatology Department, University Paris Diderot, Hôpital Lariboisière, Paris, France
  1. Correspondence to Professor Pascal Richette, Fédération de Rhumatologie, Centre Viggo Petersen, Hôpital Lariboisière, 2 rue Ambroise Paré, Paris 75010, France; pascal.richette{at}aphp.fr

Abstract

Objective To investigate the impact of systemic inhibition of interleukin 6 (IL-6) or signal transducer and activator of transcription (Stat3) in an experimental model of osteoarthritis (OA).

Methods Expression of major catabolic and anabolic factors of cartilage was determined in IL-6-treated mouse chondrocytes and cartilage explants. The anti-IL-6-receptor neutralising antibody MR16-1 was used in the destabilisation of the medial meniscus (DMM) mouse model of OA. Stat3 blockade was investigated by the small molecule Stattic ex vivo and in the DMM model.

Results In chondrocytes and cartilage explants, IL-6 treatment reduced proteoglycan content with increased production of matrix metalloproteinase (MMP-3 and MMP-13) and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS-4 and ADAMTS-5). IL-6 induced Stat3 and extracellular signal-regulated kinase (ERK) 1/2 signalling but not p38, c-Jun N-terminal kinase or Akt. In the DMM model, Stat3 was activated in cartilage, but neither in the synovium nor in the subchondral bone. Systemic blockade of IL-6 by MR16-1 alleviated DMM-induced OA cartilage lesions, impaired the osteophyte formation and the extent of synovitis. In the same model, Stattic had similar beneficial effects on cartilage and osteophyte formation. Stattic, but not an ERK1/2 inhibitor, significantly counteracted the catabolic effects of IL-6 on cartilage explants and suppressed the IL-6-induced chondrocytes apoptosis.

Conclusion IL-6 induces chondrocyte catabolism mainly via Stat3 signalling, a pathway activated in cartilage from joint subjected to DMM. Systemic blockade of IL-6 or STAT-3 can alleviate DMM-induced OA in mice.

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Footnotes

  • Handling editor Tore K Kvien

  • Contributors AL, WB, TF-B, MC-S, EH and PR contributed to the conception of the work. AL, CC, JM, H-KE, EH and PR contributed to the acquisition and interpretation of data. All authors and contributors contributed to drafting the work and critical revision of its content. All authors and contributors gave their final approval for the submitted version of the manuscript and agreed to be accountable for all aspects of this work.

  • Funding This work was supported by ART Viggo and the French Society of Rheumatology.

  • Competing interests None.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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