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AB0158 The Expression of Blimp-1 in B Cells in Peripheral Blood from Patients with SLE and Its Clinical Significance
  1. M. Wang,
  2. Y. Zhou,
  3. L. Jin,
  4. X. Fang,
  5. X. Li,
  6. G. Wang,
  7. X. Li
  1. Rheumatology and immunology, Anhui Provincial Hospital, Hefei, Anhui, PR China, Hefei, Anhui, China

Abstract

Objectives To detect the mRNA expression of B lymphocyte induced maturation protein-1 (Blimp-1) in CD19+ B cells in peripheral blood of patients with systemic lupus erythromatosus (SLE) and healthy controls, and explore its clinical significance.

Methods Expression level of Blimp-1 mRNA in CD19 +B cells in peripheral blood was determined by real-time PCR in 40 SLE patients and 20 healthy controls. The ratio of CD19CD138+ plasma cells to CD19+ B cells and the ratio of CD19+CD27+ memory B cells to CD19+ B cells were studied by flow cytometry in peripheral blood of two groups.

Results 1. The expression level of Blimp-1 mRNA in CD19+ B cells in peripheral blood od SLE patients was higher than that in healthy controls (P<0.05), and the expression level of Blimp-1 in active SLE patients was higher than that in inactive SLE patients (P<0.05). 2. The CD19CD138+ plasma cells/CD19+ B cells ratio was increased in SLE patients compared to healthy controls (P<0.01), and the CD19CD138+ plasma cells/CD19+ B cells ratio in active SLE patients group was higher than that in inactive SLE patients (P<0.05). However, the CD19+CD27+ memory B cells/CD19+ B cells ratio showed no significantly different between SLE patients and healthy controls (P>0.05). 3. The expression level of Blimp-1 was positive correlated with the CD19CD138+ plasma cells/CD19+ B cells ratio (rs=0.336, P=0.034), positive correlated with anti-dsDNA antibody and immunoglobulin G (rs=0.493, P=0.006; rs=0.527, P=0.004), positive correlated with anti-nucleosome antibody and anti-C1q antibody (rs=0.452, P=0.040; rs=0.522, P=0.015), negative related with white blood cell count and platelet count (rs= -0.367, P=0.046; rs= -0.386, P=0.035).

Conclusions The expression of Blimp-1 was up-regulated in SLE patients and significantly correlated with plasma cells, which suggests that Blimp-1 may be involved in the pathogenesis of SLE by promoting plasma cells differentiation.

Acknowledgement This work was funded by a grant from the National Nature Science Foundation of China (No. 81373187) and the Clinical Research Special Foundation of Chinese Medical Association (12040730373).

Disclosure of Interest None declared

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