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AB0148 Differential Peripheral B-Cell Phenotype in Patients with Primary Sjögren's Syndrome (PSS) Compared To Secondary Sjögren's Syndrome Associated with Systemic Lupus Erythematosus (SLE/SS)
  1. N.L. Thompson,
  2. D.A. Isenberg,
  3. E. Jury,
  4. C. Ciurtin
  1. Rheumatology, University College London, London, United Kingdom

Abstract

Background Peripheral B-cell abnormalities are a feature of both lupus (SLE) and primary Sjogren's syndrome (pSS). However, whereas patients with pSS have increased frequencies of Bm1/Bm2 (naïve), Bm2' (germinal centre founders) and Bm3–4 (centroblasts/centrocytes), but decreased Bm5 (memory) compared with healthy controls (HC), patients with SLE have increased Bm5 levels, which correlated with disease activity. No previous studies investigating the peripheral B-cell phenotype in patients with SLE and secondary SS (SLE/SS) are available.

Objectives We questioned whether the defective B-cell phenotype in pSS patients was also present in patients with SS/SLE, and whether differences in B-cell phenotype could be related to changes in B-cell lipid-raft expression and BAFF-receptor function in pSS and SLE/SS patients.

Methods Blood samples and clinical and laboratory parameters from 32 patients with pSS and SS/SLE and 13 age/sex matched HC were obtained. We used flow-cytometry to perform B-cell immunophenotyping and analysis of lipid-raft expression (marker of B-cell activation). In vitro cultures assessed lipid-raft expression in response to BAFF.

Results Patients with SLE/SS had a district B-cell phenotype, characterised by decreased Bm1 and Bm5 and increased Bm2 populations compared to HC (p=0.031, p=0.035 and p=0.01, respectively), and increased Bm2 compared to pSS (p=0.027). Bm1-cells were decreased in both pSS and SLE/SS patients compared to HC (p=0.028 and p=0.031, respectively). Both age and disease duration correlated strongly with Bm2' cells in SLE/SS patients (r=0.9572, p=0.0428), and the immunosuppressive treatment correlated negatively with the number of circulating Bm2 and Bm2' cell in pSS (r = -0.54, p=0.01 and r = -0.56, p=0.008, respectively). B-cells from patients with pSS had a significant increase in lipid-raft expression compared to HC (p=0.01) and patients with SS/SLE (p<0.05). Furthermore, lipid-raft levels correlated with BAFF-receptor expression in HC and SLE/SS B-cells (p=0.17, r=0.694) but not in pSS patients. Both disease activity score (ESSDAI) and IgG level correlated positively with lipid rafts expression in B cells from patients with pSS (r =0.79, p=0.004 and r =0.53, p=0.04, respectively).

Conclusions We showed that patients with SLE/SS had more significant B-cell abnormalities compared to HC and pSS, detectable even in a small number of patients. Also the relationship between lipid-raft and BAFF-receptor expression was altered between pSS and SLE/SS patients, and correlated with the disease activity and Ig G levels in pSS group. Further studies will validate our findings and investigate whether B-cell lipid-rafts mediate differential BAFF-receptor signalling.

  1. Bohnhorst JO, Bjorgan MB, Thoen JE, Natvig JB, Thompson KM. Bm1-Bm5 classification of peripheral blood B cells reveals circulating germinal center founder cells in healthy individuals and disturbance in the B cell subpopulations in patients with primary Sjogren's syndrome. J Immunol. 2001; 167 (7):3610–8. Epub 2001/09/21.

  2. Jacobi AM, Reiter K, Mackay M, Aranow C, Hiepe F, Radbruch A, et al. Activated memory B cell subsets correlate with disease activity in systemic lupus erythematosus: delineation by expression of CD27, IgD, and CD95. Arthritis and rheumatism. 2008; 58 (6):1762–73. Epub 2008/06/03.

Disclosure of Interest None declared

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