Article Text

PDF
AB0130 Expression of Regulatory B Cells in Peripheral Blood of Patients with Systemic Lupus Erythematosus
  1. C. Xiaoyan
  1. Department of Rheumatology, The First Municipal People's Hospital, Guangzhou 510180, China, Guangzhou, China

Abstract

Background It is generally considered that B cells regulate immune response mainly through producing specific antibodies or a variety of cytokines., but recent studies have found that the lack of B cells and CDl9 –/– mice in experimental autoimmune encephalomyelitis (EAE) mouse model is more serious, and in T cell mediated inflammation experiment, CDl9–/– mice show a more serious contact hypersensitivity (CHS). Therefore, it indicated that B cells also play an important role in the negative immune regulation.

Objectives To investigate the expression of regulatory B cells (Bregs) in peripheral blood of patients with systemic lupus erythematosus (SLE) and the function of Bregs in the pathogenesis of SLE.

Methods Thirty-eight active SLE patients, twenty-two inactive SLE patients and twenty healthy controls were enrolled in this study. The expression of IL-10+CD19+Breg and CD19+CD24hiCD38hiBreg in peripheral blood mononuclear cells (PBMC) was evaluated by flow cytometric analysis. IL-10 was measured in the culture supermatants by ELISA.

Results The expression of IL-10+CD19+Breg in PBMCs of active SLE patients was 1.54±0.64%, lower than those in inactive SLE patients (2.42±0.75)% (P<0.001) and healthy controls (4.35±1.00)% (P<0.001). And the expression of CD19+CD24hiCD38hiBreg was 1.26±0.45%, also lower than those in inactive SLE patients (2.01±0.61)% (P<0.001) and healthy controls (3.14±0.87)% (P<0.001). The level of IL-10 in the culture supermatants of active SLE patients significantly decreased, which was lower than those in inactive SLE patients (P<0.05) and healthy controls (P<0.001). The percentage of IL-10+CD19+Breg and CD19+CD24hiCD38hiBreg in peripheral blood of SLE patients was positively correlated with the level of IL-10 in the culture supermatants (r=0.652, P<0.001 and r=0.574,P<0.001).

Conclusions Expression of IL-10 related Bregs was significantly decreased in peripheral blood of active SLE patients. Bregs may play a critical role in the pathogenesis of SLE.

Disclosure of Interest None declared

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.