Article Text

PDF
AB0043 Serum Cytokine Concentrations in Inflammatory Bowel Disease (IBD) and IBD-Related Spondyloarthritis (SPA)
  1. J. Dmowska-Chalaba1,
  2. E. Kontny2
  1. 1Early Arthritis Clinic
  2. 2Department of Pathophysiology and Immunology, National Institute of Geriatrics, Rheumatology and Rehabilitation, Warsaw, Poland

Abstract

Background Inflammatory bowel disease (IBD) is a group of recurrent gastrointestinal disorders, involving Crohn disease (CD) and ulcerative colitis (UC), that is characterized by chronic mucosal inflammation and extraintestinal symptoms. Rheumatic abnormalities of a broad spectrum of symptoms, from arthralgia to spondyloarthritis (SpA), are the most common extraintestinal manifestations of IBD. The pathogenesis of IBD-related arthritis is unclear.

Objectives In this study, we analyzed the serum cytokine concentrations (interleukins: IL-10, IL-18, IL-21, IL-23, interferon γ) in patients with IBD-associated spondyloarthritis and patients with IBD not complicated with SpA symptoms.

Methods The study group consisted of 75 patients (44 female/29 male, age 43,7±12,3 year) suffering from IBD alone (n=35) or with IBD accompanied by arthritis (n=39), i.e. spondyloarthritis (IBD-SpA, n=16) or ankylosing spondylitis (IBD-AS, n=23). The control group included age and sex matched healthy volunteers (n=42). Serum cytokine concentrations were measured using commercially available kits from eBioscience, San Diego, CA, USA (Platinum ELISA for IL-18, ReadySetGo ELISAs for IL-10, IL-21, IL-23, interferon γ).

Results The concentrations of IL-23 (the mean ± SEM =156 ±14 vs 115±49 pg/ml) and IL-21 (232±79 vs 20.6 ±8 pg/ml) were significantly higher (p<0.0001) in the total group of patients than control sera. Similar significant elevation of IL-21 and IL-23 was found in IBD (146±35 and 133±40 pg/ml, respectively), IBD-SpA (124±48 and 151±37 pg/ml, respectively) and IBD-AS (194±62 and 417±228 pg/ml, respectively) groups comparing with control sera (p≤0.001). There was no significant difference between control and patients groups in the serum concentrations of other tested cytokines, except IL-18. The levels of IL-18 were significantly lower in the sera of IBD-AS group than in healthy volunteers (59±19 vs 180±36 pg/ml, p=0.02). Although we failed to show significant difference in serum cytokine concentrations between IBD and IBD-SpA or IBD-AS patients, in the IBD-AS group there was again tendency to downregulation of serum IL-18 in comparison with patients suffering from IBD alone (59±19 vs 121 ±27 pg/ml, p=0.08).

Conclusions Significant elevation of serum IL-21 and IL-23 found in IBD patients irrespectively of complication with SpA suggests implication of these cytokines in the underlying disease pathology. On the other hand, diminution of serum IL-18 in IBD-AS may point to protective action of this cytokine in AS development.

Supported by grant 2012/05/N/NZ5/02839 from the National Science Centre of Poland.

Disclosure of Interest None declared

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.