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SAT0293 Serum and Tear Cytokines and Chemokines Levels as Disease Activity Biomarkers in Patients with Primary Sjögren's Syndrome
  1. A.A. Castillo Ortiz1,
  2. R.E. Barbosa Cobos1,
  3. L.T. Becerril Mendoza1,
  4. G. Lugo Zamudio1,
  5. V. Lima Gόmez2,
  6. M.A. Moreno Eutimio3,
  7. N.G. Nieto Velázquez3,
  8. A.A. Tzec Pérez1
  1. 1Rheumatology
  2. 2Ofthalmology
  3. 3Inmunology, Hospital Juárez de México, Mexico, Mexico

Abstract

Background Evidence suggests that levels of different cytokines in primary Sjögren's syndrome (pSS) are associated with cell infiltration degree within lacrimal and salivary glands and severity of the disease. Chemokines appear to contribute to the pathogenesis of pSS.

Objectives To evaluate the association between serum and tear Th1/Th2/Th17 cytokines and chemokines leves and disease activity in patients with pSS.

Methods Case-control study, 19 patients with pSS and 13 healthy controls were included. Disease activity was assessed by EULAR Sjögren's Syndrome Disease Activity Index (ESSDAI); serum and tear IFN-γ, TNF, IL-2, IL-4, IL-6, IL-10, IL-17, IP10, MCP-1, MIG, RANTES and IL-8 levels were measured by bead-based assays following the sandwich immunoassay principle (BD Cytometric Bead Array; BD Biosciences). Differences between groups were analyzed using the Mann-Whitney U test, correlation was estimated by Spearman's correlation coefficient.

Results TNF, IL-2, IL-4, IL-6, IL-10, IP-10, MCP-1, RANTES and IL-8 tear levels were statistically different between controls and patients with pSS (p<0.005). Significant correlation (p<0.005) was showed in patients between serum IL-6 and serum IL-2 (r=0.652), IL-4 (r=0.731); serum IL-2 and serum IP-10 (r=0.640); serum IL-2 and tear IL-2 (r=0.614), IL-8 (r=0.723), IP-10 (r=0.795), MIG (r=0.755); serum IL-4 and tear IL-8 (r=0.696), MIG (r=0.707); serum IP-10 and serum MIG (r=0.847), RANTES (r=0.626); serum IP-10 and tear IP-10 (r=0.619); serum MIG and serum RANTES (r=0.719); serum MIG and tear IL-17 (r=0.619); serum RANTES and tear IL-2 (r=0.628), IL-4 (r=0.619), IL-6 (r=0.736), IL-8 (r=0.770), IP-10 (r=0.687), MCP-1 (r=0.566), MIG (r=0.740).

Conclusions Increased tear Th1/Th2 cytokines and chemokines levels were found in pSS. No correlation was found between serum or tear cytokines and chemokines levels and pSS disease activity.

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Disclosure of Interest None declared

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