Background Evidence suggests that levels of different cytokines in primary Sjögren's syndrome (pSS) are associated with cell infiltration degree within salivary and lacrimal glands and severity of the disease. Chemokines appear to contribute to the pathogenesis of pSS.
Objectives To evaluate the association between serum and salivary Th1/Th2/Th17 cytokines and chemokines leves and disease activity in patients with pSS.
Methods Case-control study, 18 patients with pSS and 13 healthy controls were included. Disease activity was assessed by EULAR Sjögren's Syndrome Disease Activity Index (ESSDAI); serum and salivary IFN-γ, TNF, IL-2, IL-4, IL-6, IL-10, IL-17, IP10, MCP-1, MIG, RANTES and IL-8 levels were measured by bead-based assays following the sandwich immunoassay principle (BD Cytometric Bead Array; BD Biosciences). Differences between groups were analyzed using the Mann-Whitney U test, correlation was estimated by Spearman's correlation coefficient.
Results IL-17, IL-2, IL-4, IFNγ, MIG and RANTES salivary levels were statistically different between controls and patients with pSS (p<0.005). Significant correlation (p<0.005) was showed in patients between serum IL-6 and serum IL-12 (r=0.004), IL-10 (r=0.006); serum IL-12 and serum RANTES (r=0.008); serum IP-10 and serum MIG (r=0.000), RANTES (r=0.003); serum MIG and serum RANTES (r=0.014); salivary IL-17 and salivary IL-2 (r=0.004), IL-4 (r=0.001), INFγ (r=0.000), TNF (r=0.002), IL-10 (r=0.000); salivary IL-6 and salivary IL-2 (r=0.000), IL-4 (r=0.000), TNF (r=0.000), IL-10 (r=0.000), IL-8 (r=0.0003), MCP-1 (r=0.007); salivary IL2 and salivary IL4 (r=0.000), INFγ (r=0.006), TNF (r=0.001), IL10 (r=0.000); salivary IL4 and sallivary TNF (r=0.001), IL10 (r=0.000), MCP-1 (r=0.009); salivary TNF and salivary INFγ (r=0.002), IL-10 (r=0.000), IP-10 (r=0.006); salivary MCP-1 and salivary TNF (r=0.000), IL-10 (r=0.001), IL-8 (r=0.003).
Conclusions Increased salivary Th1/Th2/Th17 cytokines and chemokines levels were found in pSS. No correlation was found between serum or salivary cytokines and chemokines levels and pSS disease activity.
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Disclosure of Interest None declared
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