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OP0233 Genome-Wide Pathway Analysis Reveals that VEGF Genetic Pathway Is Associated with Oral Ulcers in Systemic Lupus Erythematosus
  1. A. Julià1,
  2. P. Carreira2,
  3. R. Blanco3,
  4. V. Martínez Taboada3,
  5. L. Carreño Pérez4,
  6. J. Pérez-Venegas5,
  7. À. Olivé6,
  8. J.L. Andreu7,
  9. M. Aguirre Zamorano8,
  10. P. Vela9,
  11. J.M. Nolla10,
  12. J.L. Marenco de la Fuente11,
  13. A. Zea12,
  14. J.M. Pego13,
  15. M. Freire14,
  16. E. Díez15,
  17. A. Aterido1,
  18. A. Alonso1,
  19. M. Lόpez-Lasanta1,
  20. M. Lόpez-Corbeto1,
  21. R. Tortosa1,
  22. S. Marsal1,
  23. A. Fernández-Nebro16
  1. 1Rheumatology Research Group, Vall d'Hebron Research Institute, Barcelona
  2. 2Rheumatology Department, Hospital Universitario Doce de Octubre, Madrid
  3. 3Rheumatology Department, Hospital Universitario Marqués de Valdecilla, Santander
  4. 4Rheumatology Department, Hospital Universitario Gregorio Marañόn, Madrid
  5. 5Rheumatology Department, Hospital del SAS de Jerez de la Frontera, Cádiz
  6. 6Rheumatology Department, Hospital Universitari Germans Trias i Pujol, Badalona
  7. 7Rheumatology Department, Hospital Universitario Puerta de Hierro, Madrid
  8. 8Rheumatology Department, Hospital Universitario Reina Sofia, Cόrdoba
  9. 9Rheumatology Department, Hospital General Universitario de Alicante, Alicante
  10. 10Rheumatology Department, Hospital Universitari de Bellvitge, Barcelona
  11. 11Rheumatology Department, Hospital de Valme, Sevilla
  12. 12Rheumatology Department, Hospital Universitario Ramόn y Cajal, Madrid
  13. 13Rheumatology Department, Hospital do Meixoeiro, Vigo
  14. 14Rheumatology Department, Hospital Complejo Asistencial Universitario de Leόn, Leόn
  15. 15Rheumatology Department, Hosiptal Universitario A Coruña, A Coruña
  16. 16Rheumatology Department, Hospital Regional Universitario Carlos Haya, Málaga, Spain


Background Systemic lupus erythematosus (SLE) is a genetically complex rheumatic disease with heterogeneous clinical manifestations. Recent studies have suggested the existence of a genetic basis for the diverse SLE clinical phenotypes. Also, there is increasing evidence indicating that a substantial part of the genetic variation associated with complex diseases is explained by small-effect genes from common genetic pathways.

Objectives The objective of the present study was to identify new genetic variation associated with SLE phenotypes using a genome-wide association study at the pathway level.

Methods A total of 598,258 SNPs were genotyped in a discovery cohort of n=482 SLE patients of southern European ancestry using the Illumina platform Quad610. After quality control analysis, including ancestry estimation using principal-component analysis, genome-wide pathway analysis was performed. A total of 14 clinically relevant SLE phenotypes were tested for association with n>700 reference genetic pathways. Significantly associated pathways (corrected P-value <0.05) were subsequently tested for validation in an independent cohort of n=425 SLE patients from the same ancestry. Both discovery and validation cohort patients were Caucasian European and from Spanish origin, and were recruited by rheumatology departments from n=15 Spanish university hospitals. The validated genetic pathways were functionally characterized using in silico analysis on cell types of relevance in SLE pathogenesis.

Results In the discovery stage, two genetic pathways were significantly associated with the presence of oral ulcers and antinuclear antibodies in SLE (PFDR<0.05). In the replication stage, we validated the association between oral ulcers and vascular endothelial growth factor (VEGF) genetic pathway (P=1.3e-2). Analyzing the transcriptional effect of the topical immunotherapies used for the treatment of oral ulcers in SLE, we found a significant differential expression of VEGF pathway genes (P<0.05).

Conclusions In this work we have performed the first genome-wide association study for clinically relevant SLE phenotype using a pathway-based approach. With this new approach, we have identified and validated the association of VEGF genetic pathway with oral ulcers in SLE. These findings represent an important step towards the characterization of the genetic basis of phenotype heterogeneity in SLE.

Disclosure of Interest None declared

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