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AB0895 Effects of Colchicine on Neutrophil Apoptosis in Patients with Familial Mediterranean Fever
  1. E. Kaya1,
  2. M. Nazıroglu2,
  3. Y. Ugan3,
  4. M. Sahin3,
  5. A. Doğru3,
  6. M. Aykur4
  1. 1Department of Internal Medicine
  2. 2Neuroscience Research Center
  3. 3Department of Internal Medicine, Division of Rheumatology, Suleyman Demirel University, Faculty of Medicine, Isparta
  4. 4Department of Microbiology, Gaziosmanpasa University Faculty of Medicine, Tokat, Turkey

Abstract

Background Familial mediterranean fever (FMF) is an autoinflammatory disease associated with a self-limiting attacks of fever and serositis.

Objectives We aimed to investigate the assessment of apoptosis during acute attack and the effect of colchicine on apoptosis in patients with familial mediterranean fever (FMF).

Methods This study was performed on 7 FMF patients during acute attack period and 7 age-sex matched healthy persons. Caspase 3 and caspase 9 were studied as the markers of apoptosis. Caspase 3–9 were studied again after neutrophils were incubated with colchicine in cell culture.

Results Apoptosis level (0.21±0.009 versus 0.17±0.009, p<0.05) and markers of apoptosis (caspase 3 and 9) was significantly higher in FMF group during attack period than the control group (p<0.001). After the incubation with colchicine, apoptosis markers were found to provide a significantly decrease in comparison with attack period (p<0.001).

Table 1.

Apoptosis, caspase 3 and caspase 9 levels in between groups

Conclusions Changing the structure of pyrin after mutation can cause failure in the regulation of inflammation and apoptosis. We suggest that defects in the regulation of apoptosis may play a role in the pathogenesis of FMF. Colchicine may reduces apoptosis as well as other mechanisms of action. More studies are needed to clarify the pathogenetic mechanisms of apoptosis in FMF.

Disclosure of Interest None declared

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