Background Macrophage migration inhibitory factor (MIF) is proinflammatory, angiogenic and tissue degrading cytokine.
Objectives This study aimed to determine monosodium urate (MSU)-induced MIF production and the role of MIF in gouty arthritis.
Methods Peripheral blood and clinical data were obtained from 98 patients (31 patients with acute gouty arthritis and 67 patients with intercritical gout). Synovial fluid (SF) was obtained from patients with acute gouty arthritis and SF white blood cells and neutrophils were counted. SF and serum levels of MIF, interleukin (IL)-1, IL-8 and leukotriene B4, were measure using enzyme-linked immunosorbent assay (ELISA) and their relationship was analyzed. Peripheral blood mononuclear cells (PBMC) were isolated and cultured with MSU crystal, the production of MIF was determined.
Results SF MIF level was higher in acute gouty arthritis than osteoarthritis. Serum MIF was higher in patients with intercritical gout than patients with acute gouty arthritis or healthy volunteers. SF MIF level was positively correlated with SF WBC and neutrophil counts, IL-1b and IL-8 levels in acute gouty arthritis. Serum MIF level was correlated with IL-1b and IL-8 levels in patients with intercritical gout. MSU crystal induced MIF production in PBMC with maximal effect at 100mg/ml.
Conclusions MIF was highly produced in gouty arthritis and MSU induced MIF production. MIF could be involved in early inflammatory process in acute gouty arthritis.
Acknowledgement This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (NRF-2013R1A1A1008171).
Disclosure of Interest None declared